LRP-8 binding dual variable domain immunoglobulin proteins
AssigneeAbbVie Inc.
InventorsLorenzo Benatuil, Kangwen Deng, Denise Karaoglu Hanzatian, Maria A. Argiriadi
Life of this patent
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Abstract
The disclosure provides binding proteins that specifically bind to LRP-8 and optionally cross the blood brain barrier (BBB), localize to the brain and/or localize to the spinal cord.
Claims25
A dual variable domain immunoglobulin (DVD-Ig) binding protein capable of binding low density lipoprotein receptor-related protein 8 (LRP-8), comprising a heavy chain variable domain comprising a set of three heavy chain complementarity determining regions (CDRs) (CDR-H1, CDR-H2, and CDR-H3), and a light chain variable domain comprising a set of three light chain CDRs (CDR-L1, CDR-L2, and CDR-L3), wherein the heavy chain CDRs are:
- (a) CDR-H1:
- RFTFSNX1GMS (SEQ ID NO:
- 9), wherein X1 is F or Y, or GFTVSDYYMA (SEQ ID NO:
- 53);
- (b) CDR-H2:
- TISSGGRYTYYPDX2VKG (SEQ ID NO:
- 10), wherein X2 is S or H, or SISYEGSSTYYGDSVKG (SEQ ID NO:
- 54);
- and
- (c) CDR-H3:
- DYLYAMDY (SEQ ID NO:
- 46) or PLRYYGYNYRFAY (SEQ ID NO:
- 55), and the light chain CDRs are:
- (d) CDR-L1:
- RSSQSLVYSX3X4NTYLH (SEQ ID NO:
- 47), wherein X3 is N, T, R, W or P, and wherein X4 is G, E, L or K, or KASQNIHKNLD (SEQ ID NO:
- 56);
- (e) CDR-L2:
- KVSNRFS (SEQ ID NO:
- 48) or YTDNLQT (SEQ ID NO:
- 57);
- and
- (f) CDR-L3:
- SQSTHVPLT (SEQ ID NO:
- 49) or YQYNSGPT (SEQ ID NO:
- 58).
A dual variable domain immunoglobulin (DVD-Ig) binding protein that binds to human low density lipoprotein receptor-related protein 8 (LRP-8) at an epitope comprising amino acid residues 47-66 of human LRP-8 (SEQ ID NO:1).
A monoclonal antibody that binds to human low density lipoprotein receptor-related protein 8 (LRP-8) at an epitope comprising amino acid residues 47-66 of human LRP-8 (SEQ ID NO:1).
A monoclonal antibody capable of binding low density lipoprotein receptor-related protein 8 (LRP-8), comprising a heavy chain variable domain comprising a set of three heavy chain complementarity determining regions (CDRs) (CDR-H1, CDR-H2, and CDR-H3), and a light chain variable domain comprising a set of three light chain CDRs (CDR-L1, CDR-L2, and CDR-L3), wherein the heavy chain CDRs are:
- (a) CDR-H1:
- RFTFSNX1GMS (SEQ ID NO:
- 9), wherein X1 is F or Y, or GFTVSDYYMA (SEQ ID NO:
- 53);
- (b) CDR-H2:
- TISSGGRYTYYPDX2VKG (SEQ ID NO:
- 10), wherein X2 is S or H, or SISYEGSSTYYGDSVKG (SEQ ID NO:
- 54);
- and
- (c) CDR-H3:
- DYLYAMDY (SEQ ID NO:
- 46) or PLRYYGYNYRFAY (SEQ ID NO:
- 55),
- and the light chain CDRs are:
- (d) CDR-L1:
- RSSQSLVYSX3X4NTYLH (SEQ ID NO:
- 47), wherein X3 is N, T, R, W or P, and wherein X4 is G, E, L or K, or KASQNIHKNLD (SEQ ID NO:
- 56);
- (e) CDR-L2:
- KVSNRFS (SEQ ID NO:
- 48) or YTDNLQT (SEQ ID NO:
- 57);
- and
- (f) CDR-L3:
- SQSTHVPLT (SEQ ID NO:
- 49) or YQYNSGPT (SEQ ID NO:
- 58).
Dependencies are inferred from the claim text.