Polypeptide, and use thereof
AssigneesKaneka Corporation, National Institute of Advanced Industrial Science and Technology
InventorsTeruyuki Nishi, Kiyoshi Asai, Hiroko Hagiwara, Masayuki Machida
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Abstract
This invention provides a novel means that allows efficient secretion and production of a target protein in a host cell. This invention concerns a novel polypeptide having an activity of improving the secretion productivity of a target protein, a gene comprising a nucleotide sequence encoding such polypeptide, a vector comprising such gene, a transformant obtained by transforming a host cell with such vector, and a method for producing a protein comprising a step of culturing such transformant and recovering a target protein from the culture product.
Claims12
A method for producing a target protein comprising the steps of
- (i) culturing a transformant of a host cell comprising:
- a gene encoding the target protein operably linked to a methanol-inducible promoter, and
- a vector which comprises a gene encoding a polypeptide, wherein the polypeptide gene is
- (a) a nucleotide sequence encoding a polypeptide comprising SEQ ID NO:
- 32,
- (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence with 85% or higher sequence identity SEQ ID NO:
- 32, or
- (c) a nucleotide sequence encoding a polypeptide comprising SEQ ID NO:
- 32 with up to 5 substitutions, deletions, insertions and/or additions of amino acids;
- (ii) expressing the polypeptide and expressing the target protein;
- and
- (iii) recovering the target protein.
Dependencies are inferred from the claim text.