Dominant positive hnRNP-E1 polypeptide compositions and methods
AssigneeIndiana University Research and Technology Corporation
InventorsAsok C. Antony, Ying-Sheng Tang
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Abstract
Described herein are compositions relating to engineered hnRNP-E1 variant polypeptides, nucleic acids encoding such polypeptides, engineered hnRNP-E1 compositions, and methods of use thereof. In some embodiments, the engineered hnRNP-E1 polypeptide contains a C293S substitution and retains the ability to bind to a poly(rC)- and poly(U)-rich 5′-UTR element in its cognate mRNA targets in the absence of homocysteine. In some cases, the engineered hnRNP-E1 compositions provided herein are useful to increase the translation of a subset of mRNAs or to treat certain health conditions as described herein.
Claims13
An isolated variant of a native hnRNP-E1 polypeptide, wherein the variant hnRNP-E1 polypeptide comprises (i) at least one amino acid substitution at an amino acid residue selected from C293, C54, C158, and C201 of SEQ ID NO:1, wherein the amino acid substitution replaces a native cysteine residues with a serine residue and (ii) an RNA binding portion that binds a single-stranded RNA that comprises SEQ ID NO:6 in the absence of homocysteine and is optionally linked to a protein transduction domain.
Dependencies are inferred from the claim text.