Computationally optimized broadly reactive antigens for H1N1 influenza
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsTed M. Ross, Corey J. Crevar
Life of this patent
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Abstract
Described herein is the generation of optimized H1N1 influenza HA polypeptides for eliciting a broadly reactive immune response to H1N1 influenza virus isolates. The optimized HA polypeptides were developed through a series of HA protein alignments, and subsequent generation of consensus sequences, based on selected H1N1 viruses isolated from 1918-2011. Provided herein are optimized H1N1 HA polypeptides, and compositions, fusion proteins and VLPs comprising the HA polypeptides. Further provided are codon-optimized nucleic acid sequences encoding the HA polypeptides. Methods of eliciting an immune response against influenza virus in a subject are also provided by the present disclosure.
Claims15
An isolated nucleic acid molecule encoding a recombinant influenza hemagglutinin (HA) polypeptide, wherein the amino acid sequence of the polypeptide comprises:
- (i) no more than 5 amino acid substitutions relative to SEQ ID NO:
- 1;
- (ii) SEQ ID NO:
- 2;
- (iii) no more than 6 amino acid substitutions relative to SEQ ID NO:
- 3;
- (iv) no more than 8 amino acid substitutions relative to SEQ ID NO:
- 4;
- (v) no more than 10 amino acid substitutions relative to SEQ ID NO:
- 5;
- (vi) no more than 8 amino acid substitutions relative to SEQ ID NO:
- 6;
- (vii) no more than 10 amino acid substitutions relative to SEQ ID NO:
- 7;
- (viii) no more than 10 amino acid substitutions relative to SEQ ID NO:
- 8;
- (ix) SEQ ID NO:
- 9;
- (ix) no more than 8 amino acid substitutions relative to SEQ ID NO:
- 10;
- or
- (x) no more than 5 amino acid substitutions relative to SEQ ID NO:
- 11.
Dependencies are inferred from the claim text.