Metapneumovirus immunogens and related materials and methods
AssigneesResearch Institute At Nationwide Children's Hospital, Ohio State Innovation Foundation
InventorsJianrong Li, Yongwei Wei, Yu Zhang, Mark E. Peeples
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Abstract
The present invention provides compositions useful to induce immune response to human metapneumovirus. Also provided are related materials and methods, including methods to induce an immune response in a mammal, comprising administering the compositions herein.
Claims19
A composition comprising a live, attenuated human metapneumovirus (hMPV) comprising a mutated integrin-binding motif (RGD motif) in a fusion (F) protein of the live, attenuated hMPV, wherein the mutated RGD motif consists of a mutation or combination of mutations selected from:
- R329K;
- D331A;
- R329K and G330A;
- R329K and D331A;
- R329K and D331E;
- R329K and D331R;
- G330A and D331A;
- R329K, G330A, and D331A;
- R329K, G330A, and D331E;
- and R329K, G330A, and D331R.
A recombinant human metapneumovirus (hMPV) comprising a mutated integrin-binding motif (RGD motif) in a fusion (F) protein of the hMPV capable of inducing neutralizing antibody and a T cell immune response in a mammal without inducing clinical symptoms of hMPV infection, wherein the mutated RGD motif consists of a mutation or combination of mutations selected from R329K;
- D331A;
- R329K and G330A;
- R329K and D331A;
- R329K and D331E;
- R329K and D331R;
- G330A and D331A;
- R329K, G330A, and D331A;
- R329K, G330A, and D331E;
- and R329K, G330A, and D331R.
A method of attenuating a human metapneumovirus (hMPV) comprising mutating an integrin-binding motif (RGD motif) in a fusion (F) protein of the hMPV, wherein the RGD motif comprises an R329K mutation, or an R329K mutation and a D331A mutation.
Dependencies are inferred from the claim text.