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US 10,091,957 B2Granted
Method and apparatus for substantially isolating plant tissues
AssigneeMonsanto Technology LLC
InventorsWhitney R. Adams, Jr., Brandon Davis, Lubomyr Kucher, Brian J. Martinell, Jyoti R. Rout, Brenda A. Lowe
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Abstract
The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
Claims11
A method for preparing a plant embryo suitable for tissue culture and/or genetic transformation comprising:
- (a) placing plant seed tissue comprising seed coats and plant embryos in an aqueous environment;
- (b) contacting the tissue with an agent that selectively attaches to the embryo or seed coat;
- and
- (c) isolating at least a first embryo based on the selective attachment of the agent to the embryo or seed coat;
- wherein the agent comprises gas bubbles.
2 more elements
Dependencies are inferred from the claim text.