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US 10,087,478 B2Granted
Compositions comprising a polypeptide having cellulolytic enhancing activity and a nitrogen-containing compound and uses thereof
AssigneeNovozymes, Inc.
InventorsJason Quinlan, Feng Xu, Matthew Sweeney
Life of this patent
Estimating the term…
Abstract
The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a nitrogen-containing compound. The present invention also relates to methods of using the compositions.
Claims19
A method for producing a fermentation product, comprising:
- (a) saccharifying a cellulosic material with an enzyme composition comprising:
- (i) a GH61 polypeptide having cellulolytic enhancing activity and (ii) a nitrogen-containing compound, wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the nitrogen-containing compound enhances hydrolysis of a cellulosic material by a cellulolytic enzyme compared to the GH61 polypeptide alone or the nitrogen-containing compound alone, and wherein the nitrogen-containing compound is a compound of formula (I) or (II-A):
- wherein R1 and R2 are each independently hydrogen, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteraralkyl;
- or wherein R1 and R2 together with the attached carbon form an optionally substituted aromatic or non-aromatic ring;
- wherein R3 and R4 are each independently hydrogen, an oxygen radical, —C(O)R5, —C(O)NHR6, —C(O)OR7, —SO2R8, —OR9, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteraralkyl;
- or wherein R3 and R4, R3 and R1, or R4 and R2 together with the attached nitrogen form an optionally substituted aromatic or non-aromatic ring;
- wherein R9A, R9B, and R9C are independently hydrogen, halogen, —OH, —OR10, —CN, —NO2, —N(R11)(R12), —C(O)R13, —C(O)OR14, —C(O)NHR15, —OC(O)R16, —NHC(O)R17, —OC(O)OR18, —NHC(O)OR19, —OC(O)NHR20, —NHC(O)NHR21, —SO2R22, —SO2N(R23)(R24), —SR25, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
- and wherein two of R9A, R9B, and R9C may together form an optionally substituted fused ring;
- and
- wherein R10, R11, R12, R13, R14, R15, R16, R17, R18, R19, R20, R21, R22, R23, R24, and R25 are independently hydrogen, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
- or a salt or solvate thereof;
- or (II-3):
- 2,2,6,6-tetramethyl-1-piperidinyloxy or (II-6):
- maleamic acid;
- or a salt or solvate thereof;
- and
- wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the nitrogen-containing compound enhances hydrolysis of the cellulosic material by the enzyme composition according to the following formula with a ratio greater than 1:
- GH
-
-
- 61
-
-
- effect
- =
- %
-
-
- conversion
- (
- +
- GH
-
-
- 61
- +
- nitrogen
-
- -
-
- containing
-
-
- compound
- )
- %
-
-
- conversion
- (
- no
-
-
- GH
-
-
- 61
- +
- nitrogen
-
- -
-
- containing
-
-
- compound
- )
- ;
- (b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product;
- and
- (c) recovering the fermentation product from the fermentation.
68 more elements
A method of fermenting a cellulosic material, comprising:
- fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is saccharified with an enzyme composition comprising:
- (i) a GH61 polypeptide having cellulolytic enhancing activity and (ii) a nitrogen-containing compound, wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the nitrogen-containing compound enhances hydrolysis of a cellulosic material by a cellulolytic enzyme compared to the GH61 polypeptide alone or the nitrogen-containing compound alone, and wherein the nitrogen-containing compound is a compound of formula (I) or (II-A):
- wherein R1 and R2 are each independently hydrogen, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteraralkyl;
- or wherein R1 and R2 together with the attached carbon form an optionally substituted aromatic or non-aromatic ring;
- wherein R3 and R4 are each independently hydrogen, an oxygen radical, —C(O)R5, —C(O)NHR6, —C(O)OR7, —SO2R8, —OR9, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteraralkyl;
- or wherein R3 and R4, R3 and R1, or R4 and R2 together with the attached nitrogen form an optionally substituted aromatic or non-aromatic ring;
- wherein R9A, R9B, and R9C are independently hydrogen, halogen, —OH, —OR10, —CN, —NO2, —N(R11)(R12), —C(O)R13, —C(O)OR14, —C(O)NHR15, —OC(O)R16, —NHC(O)R17, —OC(O)OR18, —NHC(O)OR19, —OC(O)NHR20, —NHC(O)NHR21, —SO2R22, —SO2N(R23)(R24), —SR25, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
- and wherein two of R9A, R9B, and R9C may together form an optionally substituted fused ring;
- and
- wherein R10, R11, R12, R13, R14, R15, R18, R17, R18, R19, R20, R21, R22, R23, R24, and R25 are independently hydrogen, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
- or a salt or solvate thereof;
- or (II-3):
- 2,2,6,6-tetramethyl-1-piperidinyloxy or (II-6):
- maleamic acid;
- or a salt or solvate thereof;
- and
- wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the nitrogen-containing compound enhances hydrolysis of the cellulosic material by the enzyme composition according to the following formula with a ratio greater than 1:
- GH
-
-
- 61
-
-
- effect
- =
- %
-
-
- conversion
- (
- +
- GH
-
-
- 61
- +
- nitrogen
-
- -
-
- containing
-
-
- compound
- )
- %
-
-
- conversion
- (
- no
-
-
- GH
-
-
- 61
- +
- nitrogen
-
- -
-
- containing
-
-
- compound
- )
- .
65 more elements
Dependencies are inferred from the claim text.