Glycine max resistance gene(s) and use thereof to engineer plants with broad-spectrum resistance to fungal pathogens and pests

An isolated nucleic acid molecule operably linked to a heterologous promoter, said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:

• (a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
• 5, wherein said nucleotide sequence encodes a protein for Fusarium-resistance;
• (b) a nucleotide sequence having at least 95% sequence identity to the sequence of SEQ ID NO:
• 5 wherein said nucleotide sequence encodes a protein for Fusarium resistance;
• (c) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:5, wherein said nucleotide sequence encodes a protein for Fusarium-resistance;
• and wherein the nucleotide sequences of (a)-(b) contain at least one substitution modification relative to SEQ ID NO:
• 5.

A method for conferring or improving Fusarium resistance in a plant, said method comprising:

• transforming said plant with a nucleic acid molecule comprising a heterologous sequence operably linked to a promoter that induces transcription of said heterologous sequence in a plant cell;
• and regenerating stably transformed plants, wherein said heterologous sequence comprises a nucleic acid molecule that encodes one or more Fusarium resistance protein sequences selected from the group consisting of:
• Glycine max salicylic acid methyl transferase as set forth in SEQ ID NO:
• 2, Glycine max ankyrin repeat-containing protein as set forth in SEQ ID NO:
• 4, Glycine max sieve element-occlusion protein as set forth in SEQ ID NO:
• 8, Glycine max disease susceptibility 1 as set forth in SEQ ID NO:
• 6, and F. virguliforme polyamine oxidase as set forth in SEQ ID NO:
• 10, or a protein having at least 95% identity to SEQ ID NOs:
• 2, 4, 6, 8, or 10.

A method for conferring or improving Fusarium resistance of a plant, said method comprising:

• stably introducing into the genome of a plant, at least one nucleotide construct comprising a Fusarium resistance nucleic acid molecule operably linked to a heterologous promoter that drives expression in a plant cell, wherein said nucleic acid molecule encodes a polypeptide selected from the group consisting of:
• Glycine max salicylic acid methyl transferase as set forth in SEQ ID NO:
• 2, Glycine max ankyrin repeat-containing protein as set forth in SEQ ID NO:
• 4, Glycine max sieve element-occlusion protein as set forth in SEQ ID NO:
• 8, Glycine max disease susceptibility 1 (GmDS1) as set forth in SEQ ID NO:
• 6, and F. virguliforme polyamine oxidase as set forth in SEQ ID NO:
• 10, or a polypeptide having at least 95% identity to SEQ ID NOs:
• 2, 4, 6, 8, or 10.

A method of plant breeding for Fusarium resistance comprising:

• identifying a plant with a heterologous Fusarium resistance nucleic acid encoding an Glycine max salicylic acid methyl transferase as set forth in SEQ ID NO:
• 2, Glycine max ankyrin repeat-containing protein as set forth in SEQ ID NO:
• 4, Glycine max sieve element-occlusion protein as set forth in SEQ ID NO:
• 8, Glycine max disease susceptibility 1 as set forth in SEQ ID NO:
• 6, and F. virguliforme polyamine oxidase as set forth in SEQ ID NO:
• 10, or a nucleic acid encoding a protein having at least 95% identity to SEQ ID NOs:
• 2, 4, 6, 8, or 10;
• selecting said resistant plant for use a parent plant;
• crossing said parent plant with itself or a second plant, so that the Fusarium-resistance trait is passed to progeny seed;
• and
• harvesting progeny seed from said parent plant.

A modified plant with improved broad-spectrum pathogen resistance, compared to a corresponding plant with no such modification;

• said modified plant having a heterologous nucleotide sequence which includes a Fusarium resistance nucleic acid sequence, said Fusarium resistance nucleic acid sequence encoding a protein selected from the group consisting of:
• Glycine max salicylic acid methyl transferase as set forth in SEQ ID NO:
• 2, Glycine max ankyrin repeat-containing protein as set forth in SEQ ID NO:
• 4, Glycine max sieve element-occlusion protein as set forth in SEQ ID NO:
• 8, Glycine max disease susceptibility 1 (GmDS1) as set forth in SEQ ID NO:
• 6, and F. virguliforme polyamine oxidase as set forth in SEQ ID NO:10, or a protein having at least 95% identity to SEQ ID NOs:
• 2, 4, 6, 8, or 10.

A method for conferring or improving pathogen resistance in a plant, said method comprising:

• transforming said plant with a nucleic acid molecule comprising a heterologous sequence operably linked to a promoter that induces transcription of said heterologous sequence in a plant cell;
• and regenerating stably transformed plants, wherein said heterologous sequence comprises a nucleic acid molecule that encodes one or more Fusarium resistance protein sequences selected from the group consisting of:
• Glycine max salicylic acid methyl transferase as set forth in SEQ ID NO:
• 2, Glycine max ankyrin repeat-containing protein as set forth in SEQ ID NO:
• 4, Glycine max sieve element-occlusion protein as set forth in SEQ ID NO:
• 8, Glycine max disease susceptibility 1 (GmDS1) as set forth in SEQ ID NO:
• 6, and F. virguliforme polyamine oxidase as set forth in SEQ ID NO:
• 10, or a protein having at least 95% identity to SEQ ID NOs:
• 2, 4, 6, 8, or 10.

A method for conferring or improving pathogen resistance of a plant, said method comprising:

• stably introducing into the genome of a plant, at least one nucleotide construct comprising a Fusarium resistance nucleic acid molecule operably linked to a heterologous promoter that drives expression in a plant cell, wherein said nucleic acid molecule encodes a polypeptide selected from the group consisting of:
• Glycine max salicylic acid methyl transferase as set forth in SEQ ID NO:
• 2, Glycine max ankyrin repeat-containing protein as set forth in SEQ ID NO:
• 4, Glycine max sieve element-occlusion protein as set forth in SEQ ID NO:
• 8, Glycine max disease susceptibility 1 (GmDS1) as set forth in SEQ ID NO:
• 6, and F. virguliforme polyamine oxidase as set forth in SEQ ID NO:
• 10, or a polypeptide having at least 95% identity to SEQ ID NOs:
• 2, 4, 6, 8, or 10.

A method of plant breeding for Fusarium resistance comprising:

• identifying a plant with a heterologous Fusarium resistance nucleic acid encoding an Glycine max salicylic acid methyl transferase as set forth in SEQ ID NO:
• 2, Glycine max ankyrin repeat-containing protein as set forth in SEQ ID NO:
• 4, Glycine max sieve element-occlusion protein as set forth in SEQ ID NO:
• 8, Glycine max disease susceptibility 1 (GmDS1) as set forth in SEQ ID NO:
• 6, and F. virguliforme polyamine oxidase as set forth in SEQ ID NO:
• 10, or a nucleic acid encoding a protein having at least 95% identity to SEQ ID NOs:
• 2, 4, 6, 8, or 10;
• selecting said resistant plant for use a parent plant;
• crossing said parent plant with itself or a second plant, so that the Fusarium resistance trait is passed to progeny seed;
• and harvesting progeny seed from said parent plant.