DGAT genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
AssigneeE. I. Du Pont de Nemours and Company
InventorsKnut Meyer, Kevin L Stecca
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Abstract
Polynucleotide sequences encoding diacylglycerol acyltransferase genes and the use of these acyltransferases for increased seed storage lipid production and altered fatty acid profiles in oilseed plants are disclosed. Transgenic soybean seed having increased total fatty acid content of at least 20% and altered fatty acids when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. Methods for increasing the total fatty acid content of a soybean seed by at least 20% include steps of transformation and selection.
Claims20
A transgenic soybean seed having increased total fatty acid content of at least 20% and an increased protein content when compared to the total fatty acid content and protein content of a non-transgenic, null segregant soybean seed, wherein the seed comprises at least one recombinant construct having at least one diacylglycerol acyltransferase (DGAT) sequence and a construct downregulating galactinol synthase (GAS) activity wherein the DGAT sequence and the GAS construct can be in the same recombinant construct or in separate recombinant constructs.
A method for increasing the total fatty acid and protein content of a soybean seed comprising:
- (a) transforming at least one soybean cell with at least one recombinant construct having at least one diacylglycerol acyltransferase (DGAT) sequence and a construct downregulating galactinol synthase (GAS) activity wherein the DGAT sequence and the GAS construct can be in the same recombinant construct or in separate recombinant constructs;
- (b) selecting the transformed soybean cell(s) of step (a) such that plants grown from the transformed cells produce transgenic seed having an increased total fatty acid content of at least 20% and an increased protein content when compared to the total fatty acid and protein content of a non-transgenic, null segregant soybean seed.
A meal obtained from a transgenic soybean seed having increased total fatty acid content of at least 20% when compared to the total fatty acid content of a non-transgenic, null segregant soybean seed, wherein the transgenic soybean seed comprises at least one recombinant construct having at least one diacylglycerol acyltransferase (DGAT) sequence and a construct downregulating galactinol synthase (GAS) activity wherein the DGAT sequence and the GAS construct can be in the same recombinant construct or in separate recombinant constructs, further wherein protein content is increased by at least 1.5% points in the meal obtained from the transgenic soybean seed compared to the protein content of a meal obtained from the non-transgenic null segregant soybean seed.
Dependencies are inferred from the claim text.