Back to results
US 10,087,420 B2Granted
Expansion of adult stem cells in vitro
AssigneeTaiga Biotechnologies, Inc.
InventorsBrian Curtis Turner, Yosef Refaeli, Gregory Bird
Life of this patent
Estimating the term…
Abstract
Disclosed are methods for manipulating and expanding stem cell populations, including adult stem cells, the cells produced by such methods, and various protein constructs related thereto.
Claims20
A method for producing a population of conditionally immortalized adult stem cells comprising:
- a) contacting one or more adult stem cells with
- i) an exogenously synthesized Myc-fusion polypeptide that promotes one or more of cell survival or proliferation, wherein the Myc-fusion polypeptide comprises a Myc polypeptide fused to a protein transduction domain;
- and
- ii) an exogenously synthesized Bcl-2 domain polypeptide that inhibits apoptosis;
- and
- b) seeding the adult stem cells in a culture vessel so as to produce a population of conditionally immortalized adult stem cells,
- wherein adult stem cells are contacted with the Myc-fusion polypeptide and the Bcl-2 domain polypeptide prior to seeding and wherein the Myc-fusion polypeptide and the Bcl-2 domain polypeptide are cultured with the cells in the culture vessel for at least 24 hours.
4 more elements
Dependencies are inferred from the claim text.