In vitro propagation of Babesia microti
AssigneeFuller Laboratories
InventorLee Fuller
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Abstract
A method for continuous in vitro propagation of Babesia microti and microti-like species is disclosed. The method comprises incubating B. microti in culture medium comprising host erythrocytes in the presence of a source of complement and a source of anti-B. microti IgM antibody. In one embodiment, the source of anti-B. microti IgM antibody is substantially free of IgG antibody. In one embodiment, the source of complement is a cell or cell line that secretes complement.
Claims14
A method of in vitro propagation of Babesia microti, the method comprising incubation B. microti in a culture medium comprising host erythrocytes in the presence of a source of complement and a source of anti-B. microti IgM antibody, wherein the source of the anti-B. microti IgM antibody is substantially free of IgG antibody, having between about 0% to 10% IgG antibody, wherein the source of complement comprises a cell or cell line that is co-cultured with the host erythrocytes and wherein the cell or the cell line secretes the complement into the culture medium.
Dependencies are inferred from the claim text.