DNA antibody constructs and method of using same
AssigneeInovio Pharmaceuticals, Inc.
InventorsDavid Weiner, Karuppiah Muthumani, Seleeke Flingai, Niranjan Sardesai
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Abstract
The present invention relates to a composition including a recombinant nucleic acid sequence that encodes an antibody and a method of generating a synthetic antibody in a subject by administering the composition to the subject. The invention also provides a method of preventing and/or treating disease in a subject using the composition and method of generation.
Claims31
A nucleic acid molecule encoding a synthetic antibody comprising a nucleic acid sequence having at least about 95% identity over an entire length of the nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence as set forth in SEQ ID NO:67;
- (b) a nucleic acid sequence as set forth in SEQ ID NO:69;
- (c) a nucleic acid sequence as set forth in SEQ ID NO:71;
- (d) a nucleic acid sequence as set forth in SEQ ID NO:73;
- (e) a nucleic acid sequence as set forth in SEQ ID NO:75;
- (f) a nucleic acid sequence as set forth in SEQ ID NO:77;
- (g) a nucleic acid sequence as set forth in SEQ ID NO:65.
A nucleic acid molecule encoding a synthetic antibody comprising a nucleic acid sequence encoding a protein having at least about 95% identity over an entire length of the amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence as set forth in SEQ ID NO:68;
- (b) an amino acid sequence as set forth in SEQ ID NO:70;
- (c) an amino acid sequence as set forth in SEQ ID NO:72;
- (d) an amino acid sequence as set forth in SEQ ID NO:74;
- (e) an amino acid sequence as set forth in SEQ ID NO:76;
- (f) an amino acid sequence as set forth in SEQ ID NO:78;
- and
- (g) an amino acid sequence as set forth in SEQ ID NO:66.
A method of generating an anti-CHIKV synthetic antibody or anti-DENV synthetic antibody in a cell, the method comprising administering to the cell a composition comprising a recombinant nucleic acid sequence encoding an anti-CHIKV or anti-DENV antibody or an antigen binding fragment thereof, wherein the recombinant nucleic acid sequence is expressed in the cell to generate the synthetic antibody,
- wherein the anti-CHIKV or anti-DENV antibody comprises an amino acid sequence having at least about 95% identity over an entire length of the amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence as set forth in SEQ ID NO:68;
- (b) an amino acid sequence as set forth in SEQ ID NO:70;
- (c) an amino acid sequence as set forth in SEQ ID NO:72;
- (d) an amino acid sequence as set forth in SEQ ID NO:74;
- (e) an amino acid sequence as set forth in SEQ ID NO:76;
- (f) an amino acid sequence as set forth in SEQ ID NO:78;
- and
- (g) an amino acid sequence as set forth in SEQ ID NO:66.
A composition comprising two or more recombinant nucleic acid sequences, wherein each recombinant nucleic acid sequence encodes an antibody, wherein the antibody comprises an amino acid sequence having at least about 95% identity over an entire length of an amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence as set forth in SEQ ID NO:68;
- (b) an amino acid sequence as set forth in SEQ ID NO:70;
- (c) an amino acid sequence as set forth in SEQ ID NO:72;
- (d) an amino acid sequence as set forth in SEQ ID NO:74;
- (e) an amino acid sequence as set forth in SEQ ID NO:76;
- (f) an amino acid sequence as set forth in SEQ ID NO:78.
Dependencies are inferred from the claim text.