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US 10,081,812 B2Granted
D-amino acid-inducible gene expression system for Rhodosporidium and Rhodotorula
AssigneeTemasek Life Sciences Laboratory Limited
InventorsLianghui Ji, Yanbin Liu, Chong Mei John Koh
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Abstract
The present invention relates to the field of fungal biotechnology, more particularly to a strong inducible gene expression system in fungal species, such as a species of the Rhodosporidium genus or the Rhodotorula genus.
Claims20
A D-amino acid inducible gene expression system operable in a transgenic fungal cell comprising a nucleic acid construct comprising a D-amino acid inducible promoter operably linked to a heterologous polynucleotide, wherein the promoter is selected from the group consisting of:
- (a) a promoter having at least 80% identity to the nucleotide sequence set forth in SEQ ID NO:94;
- (b) a promoter having at least 80% identity to the nucleotide sequence set forth in SEQ ID NO:95;
- (c) a promoter having at least 80% identity to the nucleotide sequence set forth in SEQ ID NO:96;
- (d) a promoter having at least 80% identity to the nucleotide sequence set forth in SEQ ID NO:97;
- and
- (e) a promoter having at least 80% identity to the nucleotide sequence set forth in SEQ ID NO:5, and
- wherein the promoter comprises a promoter motif comprising the nucleotide sequence set forth in SEQ ID NO:6.
4 more elements
Dependencies are inferred from the claim text.