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AssigneeCevec Pharmaceuticals GmbH
InventorsSilke Wissing, Jens Wölfel, Nicole Faust
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The present invention relates to cell lines that are genetically modified to overexpress a β-galactoside α-2,3-sialyltransferase 1 (ST3Gal1), preferably human ST3Gal1, which can be used for the production of recombinant glycoproteins having highly or fully sialylated O-linked GalNAc glycans (GalNAc O-glycans), preferably core 1 GalNAc O-glycans, as well as to respective recombinant glycoproteins. Further, the present invention relates to respective methods of expressing recombinant glycoproteins, methods of increasing the degree of sialylation of recombinant glycoproteins, and methods of decreasing the micro-heterogeneity of GalNAc O-glycans. Finally, the present invention relates to respective uses of the above cell lines for the production of recombinant glycoproteins, for increasing the degree of sialylation of recombinant glycoproteins, and for decreasing the micro-heterogeneity of O-linked GalNAc glycans of recombinant glycoproteins.
A cell line that is genetically modified to overexpress a β-galactoside α-2,3-sialyltransferase 1 (ST3Gal1), which is further genetically modified to overexpress a β-galactoside α-2,3-sialyltransferase 4 (ST3Gal4), wherein said cell line can produce a glycoprotein comprising a sialylated GalNAc-O-glycan, said glycoprotein having an increased serum half-life as compared to a nonsialylated glycoprotein.
Dependencies are inferred from the claim text.