Universal N-glycan binding reagent
AssigneeNew England Biolabs, Inc.
InventorsMinyong Chen, Xiaofeng Shi, James C. Samuelson, Christopher H. Taron
Life of this patent
Estimating the term…
Abstract
Methods of capturing N-glycan linked glycomolecules including N-glycans, N-glycopeptides and N-glycoproteins are described. The methods provide substantially unbiased capture of charged and uncharged N-glycans and/or N-glycan linked glycomolecules. Binding reagents for substantially unbiased binding of N-glycans and/or N-glycan linked glycomolecules are also described.
Claims20
A method comprising:
- combining a binding reagent with an N-glycopeptide or N-glycan under conditions by which the binding agent selectively binds to the N-glycopeptide or N-glycan,
- wherein the binding reagent comprises an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO:
- 2 and amino acid substitutions at positions corresponding to positions 42, 43, 44, 61, and 62 in SEQ ID NO:2.
A binding reagent comprising a polypeptide comprising an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO:2 and amino acid substitutions at positions corresponding to positions 42, 43, 44, 61, and 62 in SEQ ID NO:
- 2;
- wherein the binding reagent selectively binds to a N-glycopeptide or N-glycan.
A method comprising:
- combining a binding reagent with an N-glycopeptide or N-glycan under conditions by which the binding agent selectively binds to the N-glycopeptide or N-glycan,
- wherein the binding reagent comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO:2 and a plurality of amino acid substitutions selected from positions that correspond to positions 42, 43, 44, 61, and 62 in SEQ ID NO:2.
Dependencies are inferred from the claim text.