Biological fluid collection device and biological fluid separation and testing system

A biological fluid sample separation and testing system for a multi-component blood sample, comprising:

• a biological fluid collection and separation device comprising:
• a housing defining a first chamber having a first chamber inlet and a first chamber outlet, a second chamber having a second chamber inlet and a second chamber outlet, and including a separation member disposed between the first chamber outlet and the second chamber inlet, wherein the first chamber is in communication with the second chamber inlet via the separation member, wherein the first chamber receives the multi-component blood sample;
• and
• an actuator member comprising a plunger rod and a piston including a valve,
• wherein at least a portion of the piston is located within the housing and movement of the actuator member in a first direction draws a component of the multi-component blood sample from the first chamber through the separation member to the second chamber,
• wherein the valve transitions between a closed position in which the second chamber is fully sealed, and an open position in which the second chamber is open;
• and
• a testing device having a receiving port adapted to engage the valve of the biological fluid collection and separation device, wherein the valve transitions from the closed position to the open position when the receiving port is engaged with the valve.