Back to results
US 10,080,484 B2Granted
Multispectral wide-field endoscopic imaging of fluorescence
AssigneeUniversity of Washington
InventorsChenying Yang, Eric J. Seibel, Leonard Y. Nelson, Timothy D. Soper
Life of this patent
Estimating the term…
Abstract
Improved methods, systems and apparatus relating to wide field fluorescence and reflectance imaging are provided, including improved methods, systems and apparatus relating to removal of background signals such as autofluorescence and/or fluorophore emission cross-talk; distance compensation of fluorescent signals; and co-registration of multiple signals emitted from three dimensional tissues.
Claims23
A wide field spectral imaging apparatus, comprising:
- a display;
- a wide field spectral imager configured for imaging an object with at least a reference beam and a target beam, wherein the wide field spectral imager includes:
- a first and a second fluorescence excitation light source, wherein the first fluorescence excitation light source is adapted to emit the reference beam, and wherein the second fluorescence excitation light source is adapted to emit the target beam;
- and
- a processor coupled to the wide field spectral imager, the processor comprising instructions that when executed by the processor causes the apparatus to perform operations including:
- receiving a background fluorescence signal and a target fluorescence signal respectively in response to illuminating the object with the reference beam and the target beam:
- enhancing the target fluorescence signal by subtracting the background fluorescence signal multiplied by a pre-calibrated constant ratio from the target fluorescence signal;
- and
- displaying an image of the object with the display, wherein the image is based, at least in part, on the enhanced target fluorescence signal.
6 more elements
A wide field spectral imaging method, comprising:
- receiving a target fluorescence signal and a background fluorescence signal by illuminating an object with a first and a second fluorescence excitation light source included in a wide field spectral imager;
- enhancing the target fluorescence signal by subtracting the background fluorescence signal multiplied by a pre-calibrated constant ratio from the target fluorescence signal;
- and
- displaying an image of the object based, at least in part, on the target fluorescence signal after the subtracting.
1 more element
Dependencies are inferred from the claim text.