Methods and systems for producing fungal secondary metabolites
AssigneeWisconsin Alumni Research Foundation
InventorsNancy Keller, Philipp Wiemann
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Abstract
The present invention discloses methods and systems for producing fungal secondary metabolites. The invention also discloses genetically modified organisms and kits including such organisms for producing fungal secondary metabolites.
Claims16
A genetically modified Aspergillus nidulans for producing one or more secondary metabolites comprising:
- an Aspergillus nidulans organism comprising an engineered gene cluster comprising the A. nidulans sterigmatocystin gene cluster transcriptional enhancer genes aflR and aflJ operably linked to a nitrate-inducible promoter, wherein none of the other genes included in the wild type A. nidulans sterigmatocystin gene cluster are present in the engineered gene cluster;
- wherein the genetically modified A. nidulans is capable of nitrate-induced expression of the aflR and aflJ gene products.
An expression vector for producing fungal secondary metabolites, comprising one or more Aspergillus nidulans sterigmatocystin gene cluster promoters operably linked to one or more protein-encoding genes that are not part of the A. nidulans sterigmatocystin gene cluster, wherein the one or more A. nidulans sterigmatocystin gene cluster promoters are inducible by AflR/AflJ.
Dependencies are inferred from the claim text.