US 10,077,430 B2 · Recombinant adenovirus with increased safety and anticancer activities, and use thereof

Abstract

The present invention relates to a recombinant adenovirus with increased in-vivo safety, tissue specificity, and anticancer activities, and a use thereof. Specifically, the recombinant adenovirus comprising: a promoter of the liver tissue-specific phosphoenolpyruvate carboxykinase (PEPCK) gene; a trans-splicing ribozyme which is operably linked to the promoter and acts on a cancer-specific gene; a therapeutic gene or a reporter gene which is linked to the 3′ exon of the ribozyme; and a serotype 35 fiber knob and a serotype 5 shaft, in which the orf4 gene is deleted from adenovirus E1, E3 and E4 orf1, shows remarkable in-vivo safety, high specificity for a target tissue, and remarkable anticancer effects, and thus can be useful for an anticancer drug or a cancer diagnostic agent as a gene delivery vector.

Claims8

Dependent claims

A recombinant adenovirus comprising:

a nucleic acid sequence construct comprising a liver specific phosphoenolpyruvate carboxykinase (PEPCK) gene promoter, a trans-splicing ribozyme gene operably linked to the promoter, a therapeutic gene or a reporter gene linked to the 3′ exon of the trans-splicing ribozyme gene, a gene enhancer, and a serotype 35 fiber knob and serotype 5 shaft gene, the recombinant adenovirus lacking adenovirus E1, E3 and E4 orf1 to orf4 genes,
wherein the gene enhancer is an ApoE (apolipoprotein E) gene enhancer having the nucleotide sequence of SEQ ID NO:
6, a PEPCK gene enhancer, a serum albumin gene enhancer, an AFP (alphafetoprotein) gene enhancer, a CEA (carcinoembryonic antigen) gene enhancer, or a PSA (prostate-specific antigen) gene enhancer;
and
wherein the trans-splicing ribozyme gene has the nucleotide sequence of SEQ ID NO:
3.

3 more elements

Dependencies are inferred from the claim text.

Recombinant adenovirus with increased safety and anticancer activities, and use thereof

Life of this patent

Recombinant adenovirus with increased safety and anticancer activities, and use thereof

Life of this patent

Abstract

Claims8