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US 10,076,113 B2Granted
Methods for obtaining stem cells
AssigneesInstitut Pasteur, Centre National de la Recherche Scientifique (CNRS)
InventorsFabrice Chretien, Mathilde Latil, Shahragim Tajbakhsh
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Abstract
The present invention relates to methods for obtaining stem cells from mammalian cadavers, methods for obtaining or purifying stem cells from a sample likely to contain non-stem cells, methods of regeneration of injured tissues and methods of treatment.
Claims24
A method of obtaining mammalian stem cells comprising:
- a) maintaining a biological sample comprising mammalian stem cells in a culture at an oxygen concentration equal to or less than 0.1% and at a temperature of 1-6° C. during a period of time of at least 2 days;
- and
- b) selecting viable stem cells.
A method of obtaining mammalian stem cells expressing a transgene of interest comprising:
- a) maintaining a biological material comprising mammalian stem cells in a culture at an oxygen concentration equal to or less than 0.1% and at a temperature of 1-6° C. during a period of time of at least 2 days;
- b) selecting viable stem cells;
- and
- c) transforming or transfecting the viable stem cells with a transgene of interest.
1 more element
A method of obtaining mammalian stem cells comprising:
- a) providing a culture comprising mammalian stem cells;
- b) removing oxygen from the culture to create an anoxic culture;
- c) maintaining the culture in anoxic conditions at a temperature of 1-6° C. during a period of time of at least 2 days;
- and
- d) selecting viable stem cells.
2 more elements
Dependencies are inferred from the claim text.