Compositions, methods and kits to detect herpes simplex virus nucleic acids
AssigneeGen-Probe Incorporated
InventorsDamon K. Getman, Aparna Aiyer
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Abstract
The disclosed invention is related to methods, compositions, kits and isolated nucleic acid sequences for targeting Herpes Simplex Virus (HSV) nucleic acid (e.g., HSV-1 and/or HSV-2 nucleic acid). Compositions include amplification oligomers, detection probe oligomers and/or target capture oligomers. Kits and methods comprise at least one of these oligomers.
Claims20
A method for determining the presence or absence of Herpes Simplex Virus 2 (HSV-2) in a sample, said method comprising:
- (1) contacting a sample, said sample suspected of containing HSV-2, with at least two oligomers for amplifying a target region of an HSV-2 target nucleic acid, wherein the at least two amplification oligomers comprise
- (a) a first amplification oligomer comprising a first target-hybridizing sequence that is from about 15 to about 27 contiguous nucleotides (i) contained in the sequence of SEQ ID NO:49 and that includes at least the sequence of SEQ ID NO:48 or (ii) contained in the sequence of SEQ ID NO:43 and that includes at least the sequence of SEQ ID NO:42;
- and
- (b) a second amplification oligomer comprising a second target-hybridizing sequence that is from about 15 to about 27 contiguous nucleotides (i) contained in the sequence of SEQ ID NO:51 and that includes at least the sequence of SEQ ID NO:50 or (ii) contained in the sequence of SEQ ID NO:45 and that includes at least the sequence of SEQ ID NO:44;
- (2) performing an in vitro nucleic acid amplification reaction, wherein any HSV-2 target nucleic acid present in said sample is used as a template for generating an amplification product;
- and
- (3) detecting the presence or absence of the amplification product, thereby indicating the presence or absence of HSV-2 in said sample.
A combination of at least two oligomers for determining the presence or absence of Herpes Simplex Virus 2 (HSV-2) in a sample, said oligomer combination comprising:
- first and second amplification oligomers for amplifying a target region of an HSV-2 target nucleic acid, wherein
- (a) the first amplification oligomer comprises a first target-hybridizing sequence that is from about 15 to about 27 contiguous nucleotides (i) contained in the sequence of SEQ ID NO:49 and that includes at least the sequence of SEQ ID NO:48 or (ii) contained in the sequence of SEQ ID NO:43 and that includes at least the sequence of SEQ ID NO:42;
- and
- (b) the second amplification oligomer comprises a second target-hybridizing sequence that is from about 15 to about 27 contiguous nucleotides (i) contained in the sequence of SEQ ID NO:51 and that includes at least the sequence of SEQ ID NO:50 or (ii) contained in the sequence of SEQ ID NO:45 and that includes at least the sequence of SEQ ID NO:44.
Dependencies are inferred from the claim text.