Method for producing artificial skin model, and artificial skin model
AssigneesOsaka University, Toray Industries, Inc.
InventorsMitsuru Akashi, Michiya Matsusaki, Takeshi Sakura, Koji Hashimoto, Yuji Shirakata, Satoshi Hirakawa
Life of this patent
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Abstract
Provided is a novel method capable of producing an artificial skin model. A method for producing an artificial skin model includes: providing coated cells (1), each of which is obtained by covering the surface of a cell (3) with a coating film (2) containing an extracellular matrix component; forming a dermis tissue layer (7), in which the coated cells (1) are laminated, by culturing the coated cells (1); and forming an epidermis layer (12) by arranging epidermis cells (8) on the dermis tissue layer (7).
Claims8
A production method for producing an artificial skin model, comprising:
- providing cells;
- providing, separately from the cells, a first material having an RGD sequence;
- providing, separately from the cells, a second material which interacts with the first material;
- forming extracellular matrix coated cells;
- forming a dermis tissue layer, in which the extracellular matrix coated cells are laminated, by culturing the extracellular matrix coated cells;
- and
- forming an epidermis layer by arranging epidermis cells on the dermis tissue layer,
- wherein each of the extracellular matrix coated cells comprises:
- a cell, and
- a coating film coating the cell, wherein the coating film includes the first material and the second material, and at least one of the first material and the second material includes an extracellular matrix component,
- the forming of the extracellular matrix coated cells comprises:
- forming first material coated cells by contacting the first material to the cells by at least one selected from:
- (a) soaking the cells in a liquid containing the first material,
- (b) dripping the liquid containing the first material to the cells, and
- (c) spraying the liquid containing the first material to the cells;
- and
- then contacting the second material to the first material coated cells by at least one selected from:
- (d) soaking the first material coated cells in a liquid containing the second material,
- (e) dripping the liquid containing the second material to the first material coated cells, and
- (f) spraying the liquid containing the second material to the first material coated cells,
- wherein the first material is fibronectin, and the second material is gelatin.
A production method for producing an artificial skin model, comprising:
- providing cells;
- providing, separately from the cells, a first material having an RGD sequence;
- providing, separately from the cells, a second material which interacts with the first material;
- forming extracellular matrix coated cells;
- forming a dermis tissue layer, in which the extracellular matrix coated cells are laminated, by culturing the extracellular matrix coated cells;
- and
- forming an epidermis layer by arranging epidermis cells on the dermis tissue layer,
- wherein each of the extracellular matrix coated cells comprises:
- a cell, and
- a coating film coating the cell, wherein the coating film includes the first material and the second material, and at least one of the first material and the second material includes an extracellular matrix component,
- the forming of the extracellular matrix coated cells comprises:
- forming second material coated cells by contacting the second material to the cells by at least one selected from:
- (a) soaking the cells in a liquid containing the second material,
- (b) dripping the liquid containing the second material to the cells, and
- (c) spraying the liquid containing the second material to the cells;
- and
- then contacting the first material to the second material coated cells by at least one selected from:
- (d) soaking the second material coated cells in a liquid containing the first material,
- (e) dripping the liquid containing the first material to the second material coated cells, and
- (f) spraying the liquid containing the first material to the second material coated cells,
- wherein the first material is fibronectin, and the second material is gelatin.
Dependencies are inferred from the claim text.