Method for producing terpenes
AssigneeTeknologian tutkimuskeskus VTT Oy
InventorsKirsi Bromann, Mervi Toivari, Tiina Nakari-Setälä, Laura Ruohonen
Life of this patent
Estimating the term…
Abstract
The present invention concerns a method for producing terpenes in fungi comprising the steps of (a) providing a modified terpene biosynthetic gene cluster inside a host cell, wherein one or more of the naturally occurring genes or promoters of the cluster have been replaced, truncated or removed, (b) providing a transcription factor inside the host cell, the transcription factor activating the terpene biosynthetic gene cluster; (c) cultivating said host in conditions allowing the expression of the transcription factor activating the cluster; and optionally (d) recovering the thus produced terpene product.
Claims5
Method for producing terpenes in Aspergillus nidulans, comprising the steps of:
- providing a genetically modified Aspergillus nidulans host comprising a modified terpene biosynthetic gene cluster comprising nucleic acids encoding a termpene synthase comprising the amino acid sequence of SEQ ID NO:
- 47, a HMG-CoA reductase comprising the amino acid sequence of SEQ ID NO:
- 46, and a GGPP-synthase comprising the amino acid sequence of SEQ ID NO:
- 45,
- overexpressing a nucleic acid encoding a transcription factor comprising the amino acid sequence of SEQ ID NO:
- 52, where said genetically modified Aspergillus nidulans host has improved terpene production compared to an untransformed wild type Aspergillus nidulans host,
- cultivating said host in conditions allowing the expression of the transcription factor activating the terpene biosynthetic gene cluster, and
- recovering the produced terpene product.
Dependencies are inferred from the claim text.