Microorganism having regulated expression of (R)-specific enoyl-coa hydratase gene and method for producing polyhydroxyalkanoate copolymer using same

A method for producing a polyhydroxyalkanoate (PHA) copolymer having at least 5 mol % of 3-hydroxyhexanoate (3HH) monomer, the method comprising:

• culturing a microorganism comprising a (R)-specific enoyl-CoA hydratase gene in the genome DNA, wherein a nucleotide sequence upstream of the (R)-specific enoyl-CoA hydratase gene in the genome DNA of the microorganism comprises a modification that comprises a substitution, a deletion, an insertion, and/or an addition of at least one nucleotide so that expression of the (R)-specific enoyl-CoA hydratase gene is regulated, and further comprising a polyhydroxyalkanoate synthase gene, wherein the modification comprises a modification of at least one nucleotide in a promoter sequence and/or a Shine-Dalgarno (SD) sequence originally existing upstream of the (R)-specific enoyl-CoA hydratase gene in the genome DNA;
• or an insertion or substitution of an expression regulatory DNA comprising a promoter sequence and/or SD sequence, at a site upstream of the (R)-specific enoyl-CoA hydratase gene in the genome DNA, so that the mol % of the 3HH monomer is higher than a control without the modification;
• wherein the modification comprises an insertion or substitution of an E. coli-derived promoter sequence selected from the group consisting of a trc promoter sequence, a lacUV5 promoter sequence, and a trp promoter sequence;
• and
• recovering a polyhydroxyalkanoate copolymer from the microorganism.