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US 10,072,097 B2Granted
Compositions and methods for detection of protein S-nitrosylation and oxidation
AssigneeAcademia Sinica
InventorsTzu-Ching Meng, Geen-Dong Chang, Ming-Fo Hsu
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Abstract
The present invention discloses a novel isolated antibody that specifically binds to an N-phenyl-acetamide group. Also disclosed are related compositions, kits, methods for detecting protein S-nitrosylation or oxidation vivo or in vitro, and drug screening methods.
Claims7
A method for determining the presence or absence of protein S-nitrosylation or oxidation in a sample, the method comprising:
- blocking free thiols in the sample with thiol-reactive alkylating agents selected from the group consisting of iodoacetamide (IAM), N-(4-acetylphenyl)-2-iodoacetamide (APIAM) and N-(2,3-dimethylphenyl)-2-iodoacetamide (DPIAM);
- contacting the sample with one or more reducing agents;
- reacting with a 2-Iodo-N-phenylacetamide (IAN) to form N-phenylacetamidyl cysteine;
- and
- detecting N-phenylacetamidyl cysteine by an anti-N-phenylacetamidyl cysteine (PAC) antibody, thereby determining the presence or absence of protein S-nitrosylation or oxidation.
2 more elements
Dependencies are inferred from the claim text.