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US 10,067,124 B2Granted
Immunoassay method for pro-gastrin-releasing peptide
AssigneesAbbott Laboratories, Abbott Japan Co., Ltd.
InventorsToru Yoshimura, Kenju Fujita, Barry L. Dowell
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Abstract
To provide a more convenient and more accurate method of assaying ProGRP by improving the stability of ProGRP which is known to be unstable in a biological sample. By using a blood sample in a condition in which a blood coagulation factor is not activated is used as a sample, the degradation of ProGRP is suppressed, whereby it is possible to store a sample for a long period of time and to improve the accuracy of an assay.
Claims2
A method of improving stability of Pro-Gastrin-Releasing Peptide 31-98 (ProGRP 31-98 peptide) in a serum sample comprising:
- (a) obtaining a serum sample containing a ProGRP 31-98 peptide from a subject, wherein the serum sample is not dried;
- (b) adding serine protease inhibitor phenylmethylsulfonyl fluoride (PMSF) to the serum sample to form a mixture of PMSF and the serum sample;
- (c) storing the mixture at room temperature, wherein more than 80% of ProGRP 31-98 peptide present in the mixture after step (b) remains in the mixture after 20 hours of storage at room temperature;
- whereupon the stability of the ProGRP 31-98 peptide is improved as compared to the stability in a serum sample lacking phenylmethylsulfonyl fluoride.
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Dependencies are inferred from the claim text.