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US 10,066,252 B1Granted
Compositions and methods for the biosynthesis of vanillin or vanillin beta-D glucoside
AssigneeInternational Flavors & Fragrances Inc.
InventorsEsben Halkjaer Hansen, Swee Chuang Lim Hallwyl, Klavs Riishede Hansen
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Abstract
Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to have reduced levels or activity of one or more alcohol dehydrogenases or aldehyde reductase thereby increasing the production of vanillin or vanillin beta-D-glucoside.
Claims16
A recombinant host cell having the following characteristics:
- (a) the recombinant host cell produces vanillin and/or vanillin glycoside;
- and
- (b) the recombinant host cell has a gene disruption or replacement of Alcohol Dehydrogenase 6 (ADH6) gene and a gene disruption or replacement of:
- (i) Aldehyde Reductase Intermediate 1 (ARI1) and Saccharomyces Aldehyde Reductase YGL039W genes, or
- (ii) Alcohol Dehydrogenase 7 (ADH7) and Genes de Respuesta a Estres 2 (GRE2) genes.
2 more elements
A recombinant yeast cell having the following characteristics:
- (a) the recombinant yeast cell produces vanillin and/or vanillin glycoside;
- and
- (b) the recombinant yeast cell has a gene disruption or replacement of Alcohol Dehydrogenase 6 (ADH6) gene and a gene disruption or replacement of:
- (i) Aldehyde Reductase Intermediate 1 (ARI1) and Saccharomyces Aldehyde Reductase YGL039W genes, or
- (ii) Alcohol Dehydrogenase 7 (ADH7) and Genes de Respuesta a Estres 2 (GRE2) genes.
2 more elements
A method for producing vanillin and/or vanillin glycoside comprising:
- (a) providing a recombinant host cell that produces vanillin and/or vanillin glycoside and has a gene disruption or replacement of Alcohol Dehydrogenase 6 (ADH6) gene and a gene disruption or replacement of:
- (i) Aldehyde Reductase Intermediate 1 (ARM and Saccharomyces Aldehyde, Reductase YGL039W genes, or
- (ii) Alcohol Dehydrogenase 7 (AMP) and Genes de Respuesta a Estres 2 (GRE2) genes;
- (b) cultivating said recombinant host cell for a time sufficient for said recombinant host cell to produce vanillin and/or vanillin glycoside;
- and
- (c) isolating vanillin and/or vanillin glycoside from said recombinant host cell or from the cultivation supernatant, thereby producing vanillin and/or vanillin glycoside.
3 more elements
Dependencies are inferred from the claim text.