Yeast cells having NADP(H)-dependent reductive TCA pathway from pyruvate to succinate
AssigneeCargill, Incorporated
InventorsBrian J. Rush, Kevin T. Watts, Vernon L. McIntosh, Arlene M. Fosmer, Gregory M. Poynter, Thomas W. McMullin
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Abstract
Recombinant yeast cells contain a reductive TCA pathway from phosphoenolpyruvate or pyruvate to succinate. At least one metabolic step in the pathway includes a reaction of NADPH to produce NADP+. The yeast cell contains at least one exogenous NADPH-dependent gene in the pathway from phosphoenolpyruvate or pyruvate to succinate, preferably an NADPH-dependent malate dehydrogensase or fumarate reducase gene (or both).
Claims4
A recombinant I. orientalis cell having an active reductive TCA pathway from pyruvate to succinate and which further metabolizes succinate to one or more succinate metabolization products, which reductive TCA pathway includes a reaction that oxidizes NADPH to NADP+ comprising a conversion of fumarate to succinate catalyzed by an NADPH-dependent fumarate reductase enzyme and the recombinant cell has integrated into its genome an heterologous fumarate reductase gene that encodes for the NADPH-dependent fumarate reductase enzyme.
Dependencies are inferred from the claim text.