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US 10,066,240 B2Granted
Nucleic acid vectors and uses thereof
AssigneeSymvivo Corporation
InventorsHerbert Alexander Graves, Mark Andrew Fox, Sheetal Raithatha, Umesh Ramachandran
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Abstract
There are disclosed nucleic acid vectors for use in both gram positive and gram negative bacteria. In embodiments the vectors comprise a prokaryotic expression cassette and in embodiments comprise a eukaryotic expression cassette. In embodiments the vectors encode a hybrid protein comprising a DNA binding domain, a CPP domain and a signal sequence.
Claims13
A DNA plasmid comprising:
- a prokaryotic expression cassette for expression in a Bifidobacteria sp. bacterium of a hybrid protein comprising at least one DNA binding domain, at least one cell penetrating peptide (CPP) domain C-terminal relative to the at least one DNA binding domain, and at least one Bifidobacterium secretion signal sequence directing secretion of a protein:DNA plasmid complex from the Bifidobacteria sp. bacterium, wherein the at least one Bifidobacterium secretion signal sequence is N-terminal in the hybrid protein relative to the at least one DNA binding domain;
- at least one DNA motif recognized by the at least one DNA binding domain of the hybrid protein;
- and
- a eukaryotic expression cassette which expresses a cargo sequence in a eukaryotic cell.
1 more element
Dependencies are inferred from the claim text.