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US 10,066,209 B2Granted
Isolation and cultivation of stem/progenitor cells from the amniotic membrane of umbilical cord and uses of cells differentiated therefrom
AssigneeCellresearch Corporation Pte. Ltd.
InventorToan-Thang Phan
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Abstract
The present invention relates to the generation of a chondrocyte using mesenchymal stem/progenitor cells obtained from the amniotic membrane of umbilical cord and therapeutic uses of such chondrocytes.
Claims4
A method of generating a chondrocyte comprising:
- placing umbilical cord amniotic membrane mesenchymal stem cells (UCMC) in a container;
- culturing the UCMC in a growth medium allowing cell proliferation of the UCMC without differentiation of the UCMC;
- and
- culturing the UCMC in a growth medium for differentiating the UCMC into chondrocytes comprising insulin, transferrin, selenous acid, epidermal growth factor, transforming growth factor-β3, dexamethasone, ascorbic acid, sodium pyruvate, and proline to differentiate the UCMC into a chondrocyte.
1 more element
Dependencies are inferred from the claim text.