In vivo induced toxoplasma gondii protein for application in diagnosis, vaccine and therapy
AssigneeUniversiti Sains Malaysia
InventorsRahmah Binti Noordin, Atefeh Amerizadeh
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Abstract
The present invention relates to methods of screening biological samples for the presence of T. gondii. More particularly, the present invention relates to a sensitive and specific screening test for the presence of Toxoplasmosis in subjects by using or detecting the in vivo-induced T. gondii RAP domain binding protein antigen. The invention further relates to the use of the in vivo-induced antigen in the prevention or therapy of Toxoplasmosis.
Claims8
A method of detecting T. gondii in a biological sample, comprising the steps of:
- a) providing at least one biological sample;
- and
- b) i) contacting the at least one biological sample with an antigen, wherein the antigen is an isolated or recombinant T. gondii RAP domain-containing protein comprising the amino acid sequence shown in SEQ ID NO:
- 2 or SEQ ID NO:
- 4 or an antigenic fragment thereof;
- and detecting specific and selective antigen-antibody binding between saki antigen and T. gondii-specific antibody, wherein the binding indicates the presence of T. gondii in the sample;
- or
- ii) detecting the presence of T. gondii RAP domain-containing protein, or nucleic acid molecule encoding same which comprises the sequence shown in SEQ ID NO:
- 1 or SEQ ID NO:
- 3, in the biological sample, wherein the detection of the RAP domain-containing protein or nucleic acid molecule in the biological sample constitutes detection of T. gondii.
A method of detecting whether a subject has toxoplasmosis,
- comprising the steps;
- a) contacting a sample from the subject with an antigen, wherein the antigen is an isolated or recombinant T. gondii RAP domain-containing protein comprising the amino acid sequence shown in SEQ ID NO:
- 2 or SEQ ID NO:
- 4 or an antigenic fragment thereof, and detecting specific and selective antibody-antigen binding, wherein the binding indicates the presence of T. gondii-specific antibodies and toxoplasmosis in the subject;
- or
- b) detecting the presence of T. gondii RAP domain-containing protein, or nucleic acid molecule encoding same which comprises the sequence shown in SEQ ID NO:
- 1 or SEQ ID NO:
- 3, in a sample from the subject, wherein the detection of the RAP domain-containing protein or nucleic acid molecule in the sample indicates the presence of toxoplasmosis.
A method of treatment or prophylaxis of toxoplasmosis, comprising administering to a subject an efficacious amount of a vaccine comprising at least one T. gondii RAP domain-containing protein or antigenic fragment thereof, wherein the RAP domain-containing protein comprises the amino acid sequence shown in SEQ ID NO:
- 2 or SEQ ID NO:
- 4.
A method of treating toxoplasmosis in a subject in need thereof, comprising administering to the subject at least one T. gondii RAP domain-containing protein comprising the amino acid sequence shown in SEQ ID NO:
- 2 or SEQ ID NO:
- 4 or an antigenic fragment thereof.
Dependencies are inferred from the claim text.