Genes and proteins for aromatic polyketide synthesis
AssigneesNational Research Council of Canada, University of Saskatchewan
InventorsJonathan E. Page, Steve Gagne
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Abstract
Nucleic acid molecules encoding polypeptides having polyketide synthase activity have been identified and characterized. Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds in organisms. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.
Claims9
A process of synthesizing a polyketide comprising:
- reacting an alkanoyl CoA with malonyl CoA in presence of a type III polyketide synthase enzyme and a polypeptide with at least 95% sequence identity to SEQ ID NO:
- 2 or that is a conservatively substituted amino acid sequence of the sequence set forth in SEQ ID NO:
- 2 and having polyketide cyclase activity.
A process of synthesizing a naturally-occurring cannabinoid compound or a non-naturally occurring analog of a cannabinoid compound in an organism, cell or tissue comprising expressing a nucleic molecule that encodes a polypeptide with at least 95% sequence identity to SEQ ID NO:
- 2 or that is a conservatively substituted amino acid sequence of the sequence set forth in SEQ ID NO:
- 2 and having polyketide cyclase activity, in the organism, cell or tissue in the presence of a type III polyketide synthase enzyme, an alkanoyl CoA and malonyl CoA.
Dependencies are inferred from the claim text.