Capsule array devices and methods of use
Assignee10x Genomics, Inc.
InventorsBenjamin Hindson, Serge Saxonov, Michael Schnall-Levin
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Abstract
This disclosure provides microwell capsule array devices. The microwell capsule array devices are generally capable of performing one or more sample preparation operations. Such sample preparation operations may be used as a prelude to one more or more analysis operations. For example, a device of this disclosure can achieve physical partitioning and discrete mixing of samples with unique molecular identifiers within a single unit in preparation for various analysis operations. The device may be useful in a variety of applications and most notably nucleic-acid-based sequencing, detection and quantification of gene expression and single-cell analysis.
Claims30
A composition for use in barcoding nucleic acid molecules, comprising:
- a population of at least 1,000 gel beads comprising a plurality of nucleic acid barcode molecules,
- wherein a given gel bead of said at least 1,000 gel beads comprises at least 1,000 nucleic acid barcode molecules of said plurality of nucleic acid barcode molecules,
- wherein at least (i) a first subset of said at least 1,000 nucleic acid barcode molecules is coupled to an interior surface of said given gel bead, and (ii) a second subset of said at least 1,000nucleic acid barcode molecules is coupled to an outer surface of said given gel bead, and
- wherein each of said at least 1,000 nucleic acid barcode molecules comprises an identical barcode sequence that differs from barcode sequences of barcode molecules comprised in other gel beads of said at least 1,000 gel beads.
A composition for use in barcoding a nucleic acid molecule, comprising:
- at least 1,000 nucleic acid barcode molecules coupled to a gel bead, wherein said gel bead is degradable upon application of a stimulus,
- wherein at least a subset of said at least 1,000 nucleic acid barcode molecules is coupled to an interior surface of said gel bead, and
- wherein each of said at least 1,000 nucleic acid barcode molecules coupled to said gel bead comprises an identical barcode sequence.
Dependencies are inferred from the claim text.