Heterologous production of patchoulol, β-santalene, and sclareol in moss cells
AssigneesUniversity of Copenhagen, Firmenich SA
InventorsHenrik Simonsen, Dong-Fang Chen, Xin Zhan, Xiwu Pan, Brian Christopher King
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Abstract
The present invention relates to methods for preparing patchoulol, β-santalene, and sclareol in transgenic moss cells that include heterologous nucleic acid molecules encoding a polypeptide or synthase capable of using FPP or GGPP as a substrate. Methods for producing the transgenic moss cell, as well as the transgenic moss cell itself are also disclosed.
Claims8
A transgenic moss plant or moss cell capable of producing farnesyl-pyrophosphate (FPP) or geranylgeranyl-pyrophosphate (GGPP), wherein the transgenic moss plant or moss cell comprises a heterologous nucleic acid molecule encoding a polypeptide having a patchoulol synthase activity to catalyze the conversion of FPP to patchoulol, wherein the polypeptide having a patchoulol synthase activity comprises an amino acid sequence having at least 94% sequence identity to SEQ ID NO:
- 17, wherein the transgenic moss plant or moss cell is Physcomitrella patens, and wherein the transgenic moss plant or moss cell emits volatile patchoulol.
A method for producing a transgenic moss plant or moss cell that emits volatile patchoulol comprising introducing into a moss cell capable of producing farnesyl-pyrophosphate (FPP) or geranylgeranyl-pyrophosphate (GGPP) a heterologous nucleic acid molecule encoding a polypeptide having a patchoulol synthase activity to catalyze the conversion of FPP to patchoulol, and optionally regenerating a transgenic moss plant from the moss cell;
- wherein the polypeptide having a patchoulol synthase activity comprises an amino acid sequence having at least 94% sequence identity to SEQ ID NO:
- 17, wherein the transgenic moss plant or moss cell is Physcomitrella patens.
Dependencies are inferred from the claim text.