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US 10,053,684 B2Granted
Xylose isomerase signature sequences
AssigneeBP Corporation North America Inc.
InventorsDavid Neal Nunn, Jr., Peter Luginbuhl, Ling Li, Adam Martin Burja, Jon Peter Flash Bartnek
Life of this patent
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Abstract
This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.
Claims16
A vector comprising a nucleic acid which encodes a polypeptide comprising a xylose isomerase signature sequence, wherein the xylose isomerase signature sequence is selected from the group consisting of SEQ ID NO:
- 212, SEQ ID NO:
- 213 and SEQ ID NO:
- 214 or a combination thereof,
- wherein for SEQ ID NO:212:
- Amino acid residue 3 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine,
- Amino acid residue 6 is Arginine or Lysine;
- Amino acid residue 7 is any 2 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 9 is Tryptophan, Tyrosine or Phenylalanine;
- Amino acid residue 10 is Aspartic Acid, Asparagine or Glycine;
- Amino acid residue 11 is any 2 or 3 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 12 is Aspartic Acid, Asparagine, Glutamic Acid, Glycine or Threonine;
- and
- Amino acid residue 13 is Alanine or Threonine;
- wherein for SEQ ID NO:213:
- Amino acid residue 3 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 6 is Arginine or Lysine;
- Amino acid residue 7 is any 2 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 9 is Tryptophan, Tyrosine or Phenylalanine;
- Amino acid residue 10 is Aspartic Acid, Asparagine or Glycine;
- Amino acid residue 11 is any 3 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 12 is Alanine, Arginine, Asparagine, Aspartic acid, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- and
- Amino acid residue 13 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- and
- wherein for SEQ ID NO:214:
- Amino acid residue 1 is Phenylalanine or Tryptophan;
- Amino acid residue 4 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 6 is Leucine, Valine or Isoleucine;
- Amino acid residue 7 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 8 is Aspartic Acid or Glutamic Acid;
- Amino acid residue 11 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 12 is Threonine, Serine or Asparagine;
- Amino acid residue 13 is Isoleucine or Valine;
- and
- Amino acid residue 14 is Glutamic acid or Alanine.
32 more elements
A recombinant cell which expresses the polypeptide comprising a xylose isomerase signature sequence, wherein the xylose isomerase signature sequence is selected from the group consisting of SEQ ID NO:
- 212, SEQ ID NO:
- 213 and SEQ ID NO:
- 214 or a combination thereof,
- wherein for SEQ ID NO:212:
- Amino acid residue 3 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine,
- Amino acid residue 6 is Arginine or Lysine;
- Amino acid residue 7 is any 2 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 9 is Tryptophan, Tyrosine or Phenylalanine;
- Amino acid residue 10 is Aspartic Acid, Asparagine or Glycine;
- Amino acid residue 11 is any 2 or 3 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 12 is Aspartic Acid, Asparagine, Glutamic Acid, Glycine or Threonine;
- and
- Amino acid residue 13 is Alanine or Threonine;
- wherein for SEQ ID NO:213:
- Amino acid residue 3 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 6 is Arginine or Lysine;
- Amino acid residue 7 is any 2 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 9 is Tryptophan, Tyrosine or Phenylalanine;
- Amino acid residue 10 is Aspartic Acid, Asparagine or Glycine;
- Amino acid residue 11 is any 3 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 12 is Alanine, Arginine, Asparagine, Aspartic acid, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- and
- Amino acid residue 13 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- and
- wherein for SEQ ID NO:214:
- Amino acid residue 1 is Phenylalanine or Tryptophan;
- Amino acid residue 4 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 6 is Leucine, Valine or Isoleucine;
- Amino acid residue 7 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 8 is Aspartic Acid or Glutamic Acid;
- Amino acid residue 11 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
- Amino acid residue 12 is Threonine, Serine or Asparagine;
- Amino acid residue 13 is Isoleucine or Valine;
- and
- Amino acid residue 14 is Glutamic acid or Alanine.
32 more elements
Dependencies are inferred from the claim text.