Microwave-assisted peptide synthesis
AssigneeCEM Corporation
InventorsJonathan McKinnon Collins, Joseph Joshua Lambert, Michael John Collins
Life of this patent
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Abstract
An instrument and method for accelerating the solid phase synthesis of peptides are disclosed. The method includes the steps of deprotecting a protected first amino acid linked to a solid phase resin by admixing the protected linked acid with a deprotecting solution in a microwave transparent vessel while irradiating the admixed acid and solution with microwaves, activating a second amino acid, coupling the second amino acid to the first acid while irradiating the composition in the same vessel with microwaves, and cleaving the linked peptide from the solid phase resin by admixing the linked peptide with a cleaving composition in the same vessel while irradiating the composition with microwaves.
Claims8
An instrument for microwave-assisted solid phase peptide synthesis comprising:
- a source of microwave radiation;
- a cavity that supports a single mode at frequencies produced by said source;
- a microwave transparent reaction vessel in said cavity
- a source of inert gas for agitating compositions in said vessel in said cavity;
- a plurality of amino acid containers;
- and
- a processor that controls the addition of amino acids to said vessel at appropriate points in a solid phase peptide synthesis.
Dependencies are inferred from the claim text.