Method for estimating an amount of analyte in a fluid
AssigneesCommissariat A L'energie Atomique Et Aux Energies Alternatives, Avalun
InventorsJean-Guillaume Coutard, Patrick Pouteau, Myriam Laure Cubizolles
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Abstract
The invention is a method for estimating the amount of analyte in a fluid sample, and in particular in a bodily fluid. The sample is mixed with a reagent able to form a color indicator in the presence of the analyte. The sample is then illuminated by a light beam produced by a light source; an image sensor forms an image of the beam transmitted by the sample, from which image a concentration of the analyte in the fluid is estimated. The method is intended to be implemented in compact analyzing systems. One targeted application is the determination of the glucose concentration in blood.
Claims19
A method for estimating an amount of an analyte in a bodily fluid sample containing particles which is in a holder outside a body, comprising:
- a) mixing the sample with a first reagent able to form a color indicator in the presence of said analyte,
- b) following this mixing, illuminating the sample using a light source able to emit light toward the sample,
- c) acquiring, using an image sensor, an image of light transmitted or reflected by the sample, and
- d) estimating, depending on said image, an amount of said analyte, by:
- i) selecting a measuring zone in said image, said measuring zone comprising a plurality of pixels,
- ii) determining, inside the measuring zone, a region of interest and at least one region of exclusion corresponding to a trace of air bubbles, and
- iii) estimating the amount of analyte from a quantity representative of the intensity of the pixels in said region of interest, without taking into account the intensity of the pixels of each region of exclusion,
- the method also including mixing the sample with a lysis reagent able to lyse said particles resulting in formation of the air bubbles.
A method for estimating an amount of glucose in a bodily fluid sample containing red blood cells which is in a holder outside a body, comprising:
- e) mixing the sample with a first reagent able to form a color indicator in the presence of glucose,
- f) following this mixing, illuminating the sample using a light source able to emit light toward the sample,
- g) acquiring, using an image sensor, an image of light transmitted or reflected by the sample, and
- h) estimating, depending on said image, an amount of glucose, by:
- iv) selecting a measuring zone in said image, said measuring zone comprising a plurality of pixels,
- v) determining, inside the measuring zone, a region of interest and at least one region of exclusion corresponding to a trace of air bubbles, and
- vi) estimating the amount of glucose from a quantity representative of the intensity of the pixels in said region of interest, without taking into account the intensity of the pixels of each region of exclusion,
- the method also including mixing the sample with a lysis reagent able to lyse said red blood cells resulting in formation of the air bubbles.
Dependencies are inferred from the claim text.