Back to results
US 10,047,120 B2Granted
Method for producing a composition for treating a tooth lesion
AssigneeCredentis AG
InventorsMichael Hug, Dominikus Amadeus Lysek
Life of this patent
Estimating the term…
Abstract
The invention relates to a method for preparing a composition for treating a tooth lesion, said composition comprising peptides that are capable of undergoing self-assembly at a certain pH. The compositions of the invention are highly suitable for being used in the medical field, in particular for remineralizing a tooth lesion such as a subsurface caries lesion.
Claims31
A composition in lyophilized form comprising
- a peptide that undergoes self-assembly at a pH value below 7.5, wherein the peptide is predominantly in a monomeric form;
- a non-reducing sugar;
- and
- a non-volatile buffer;
- wherein upon reconstitution of the composition in water, a solution with an ionic strength of less than 0.15 having a pH that is 0.1 to 0.5 pH units above the pH at which the peptide starts to undergo self-assembly is obtained in which the peptide is predominantly present in a monomeric form.
2 more elements
A method for producing a composition suitable for treating a tooth lesion, comprising
- a) providing a solution comprising a peptide that starts to undergo self-assembly at a pH below 7.5, wherein said solution comprises a buffer which is non-volatile during lyophilisation and which provides a pH which is 0.1 to 0.5 pH units above the pH at which the peptide starts to undergo self-assembly;
- b) adding a compound that increases the pH of the solution to 8.0 or higher, said compound being sufficiently volatile to be removed during lyophilization;
- and
- c) adding a non-reducing sugar;
- d) lyophilizing the solution to produce a lyophilisate;
- and
- e) dissolving the lyophilizate in water to obtain a solution with an ionic strength of less than 0.15 having a pH that is 0.1 to 0.5 pH units above the pH at which the peptide starts to undergo self-assembly, said solution comprising the peptide predominantly in its monomeric state.
4 more elements
Dependencies are inferred from the claim text.