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US 10,041,116 B2Granted
Methods and systems for processing polynucleotides
Assignee10x Genomics, Inc.
InventorsBenjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov
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Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
Claims30
A method for identifying an analyte from a single cell, comprising:
- (a) causing an analyte of said single cell to contact one or more antibodies, thereby providing an antibody from said one or more antibodies coupled to said analyte, wherein said antibody is associated with a first nucleic acid molecule comprising a first barcode sequence;
- (b) using said first nucleic acid molecule and a second nucleic acid molecule to generate a third nucleic acid molecule, wherein said second nucleic acid molecule comprises a second barcode sequence, and wherein said third nucleic acid molecule comprises (i) said first barcode sequence or a complement thereof, and (ii) said second barcode sequence or a complement thereof;
- (c) processing said third nucleic acid molecule or a derivative thereof to identify said first barcode sequence and said second barcode sequence;
- and
- (d) using said first barcode sequence and said second barcode sequence identified in (c) to identify said analyte of said single cell.
2 more elements
Dependencies are inferred from the claim text.