Targeted sequencing of biomolecules by pulling through a liquid-liquid interface with an atomic force microscope

An apparatus for sequencing a polymer, the apparatus comprising:

• a computer comprising a processor for executing software;
• a chamber filled with a first medium and a second medium on top of the first medium, such that the polymer is associated with the first and second mediums, the first medium comprising water, the second medium comprising oil;
• an atomic force microscope coupled to the computer;
• a tip of the atomic force microscope attached to one end of the polymer of the blocks;
• and
• an opposite end of the polymer of the blocks attached to a bottom surface of the chamber in the first medium comprising the water;
• wherein the atomic force microscope, via the computer, is operable to:
• traverse the polymer from the first medium to the second medium, wherein the polymer comprises blocks;
• as the polymer traverses from the first medium to the second medium, measure different measured forces corresponding to each of the blocks;
• and
• distinguish the blocks from one another according to the different measured forces measured for each of the blocks as each of the blocks traverses from the first medium to the second medium.