Plants having increased tolerance to herbicides
AssigneeBASF Agro B.V.
InventorsRaphael Aponte, Stefan Tresch, Matthias Witschel, Jens Lerchl, Jill Marie Paulik, Chad Brommer
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Abstract
The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a benzoxazinone-derivative herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.
Claims48
A method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of:
- a) providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a herbicide-tolerant mutant protoporphyrinogen oxidase (PPO) enzyme comprising an amino acid substitution at a position corresponding to position Leu397 of SEQ ID NO:2 and an amino acid substitution at a position corresponding to position Phe420 of SEQ ID NO:2, wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate, glutamate, or glutamine, and wherein the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine or methionine;
- and
- b) applying to said site an effective amount of a benzoxazinone-derivative herbicide.
An isolated nucleic acid encoding a herbicide-tolerant mutant PPO polypeptide comprising an amino acid substitution at a position corresponding to position Leu397 of SEQ ID NO:2 and an amino acid substitution at a position corresponding to position Phe420 of SEQ ID NO:2, wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate, glutamate, or glutamine, and wherein the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine or methionine.
A herbicide-tolerant mutant PPO enzyme comprising an amino acid substitution at a position corresponding to position Leu397 of SEQ ID NO:2 and an amino acid substitution at a position corresponding to position Phe420 of SEQ ID NO:2, wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate, glutamate, or glutamine, and wherein the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine or methionine.
A plant that expresses a mutagenized or recombinant herbicide-tolerant mutant PPO polypeptide comprising an amino acid substitution at a position corresponding to position Leu397 of SEQ ID NO:2 and an amino acid substitution at a position corresponding to position Phe420 of SEQ ID NO:2, wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate, glutamate, or glutamine, and wherein the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine or methionine.
A method of producing a transgenic plant cell with an increased tolerance to a benzoxazinone-derivative herbicide as compared to a wild type variety of the plant cell comprising transforming the plant cell with an expression cassette comprising a nucleic acid encoding a herbicide-tolerant mutant PPO polypeptide comprising an amino acid substitution at a position corresponding to position Leu397 of SEQ ID NO:2 and an amino acid substitution at a position corresponding to position Phe420 of SEQ ID NO:2, wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate, glutamate, or glutamine, and wherein the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine or methionine.
A method of producing a transgenic plant comprising (a) transforming a plant cell with an expression cassette comprising a nucleic acid encoding a herbicide-tolerant mutant PPO polypeptide comprising an amino acid substitution at a position corresponding to position Leu397 of SEQ ID NO:2 and an amino acid substitution at a position corresponding to position Phe420 of SEQ ID NO:2, wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate, glutamate, or glutamine, and wherein the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine or methionine, and (b) generating a plant with an increased resistance to benzoxazinone-derivative herbicide from the plant cell.
A method of identifying or selecting a transformed plant cell, plant tissue, plant or part thereof comprising:
- i) providing a transformed plant cell, plant tissue, plant or part thereof, wherein said transformed plant cell, plant tissue, plant or part thereof comprises a polynucleotide encoding a herbicide-tolerant mutant PPO enzyme that is used as a selection marker, wherein the herbicide-tolerant mutant PPO enzyme comprises an amino acid substitution at a position corresponding to position Leu397 of SEQ ID NO:2 and an amino acid substitution at a position corresponding to position Phe420 of SEQ ID NO:2, wherein the amino acid corresponding to position Leu397 of SEQ ID NO:2 is substituted with aspartate, glutamate, or glutamine, wherein the amino acid corresponding to position Phe420 of SEQ ID NO:2 is substituted with valine or methionine, and wherein said transformed plant cell, plant tissue, plant or part thereof may comprise a further isolated polynucleotide;
- ii) contacting the transformed plant cell, plant tissue, plant or part thereof with at least one benzoxazinine-derivative compound;
- iii) determining whether the plant cell, plant tissue, plant or part thereof is affected by the inhibiting compound;
- and iv) identifying or selecting the transformed plant cell, plant tissue, plant or part thereof.
Dependencies are inferred from the claim text.