Polypeptides having mannanase activity and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsKristian Bertel Roemer M. Krogh, Nikolaj Spodsberg, Klaus Gori, Pernille Von Freiesleben
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Abstract
The present invention relates to polypeptides having mannanase activity, catalytic domains, and carbohydrate binding modules, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding modules.
Claims16
A method for degrading mannan, comprising applying a composition to the mannan, the composition including a polypeptide selected from the group consisting of:
- (a) a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 3 or the mature polypeptide of SEQ ID NO:
- 2;
- and
- (b) a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 6 or the mature polypeptide of SEQ ID NO:
- 5.
A method for producing a coffee extract, comprising the steps:
- (a) providing roast and ground coffee beans;
- (b) adding to the coffee beans, water and a polypeptide;
- (c) incubating to make an aqueous coffee extract;
- and
- (d) separating the coffee extract from the extracted coffee beans;
- the polypeptide of step (b) selected from the group consisting of:
- (i) a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 3 or the mature polypeptide of SEQ ID NO:
- 2;
- and
- (ii) a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 6 or the mature polypeptide of SEQ ID NO:
- 5.
A process for degrading a cellulosic material, comprising:
- treating the cellulosic material with an enzyme composition in the presence of a polypeptide selected from the group consisting of:
- (a) a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 3 or the mature polypeptide of SEQ ID NO:
- 2;
- and
- (b) a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 6 or the mature polypeptide of SEQ ID NO:
- 5.
A process for producing a fermentation product, comprising:
- (a) saccharifying a cellulosic material with an enzyme composition in the presence of a polypeptide;
- (b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product;
- and
- (c) recovering the fermentation product from the fermentation;
- the polypeptide of step (a) selected from the group consisting of:
- (i) a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 3 or the mature polypeptide of SEQ ID NO:
- 2;
- and
- (ii) a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 6 or the mature polypeptide of SEQ ID NO:
- 5.
A process of fermenting a cellulosic material, comprising:
- fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is saccharified with an enzyme composition in the presence of a polypeptide selected from the group consisting of:
- (a) a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 3 or the mature polypeptide of SEQ ID NO:
- 2;
- and
- (b) a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 6 or the mature polypeptide of SEQ ID NO:
- 5.
A nucleic acid construct or expression vector, comprising a polynucleotide encoding a polypeptide operably linked to one or more control sequences that direct the production of the polypeptide in an expression host;
- the polypeptide selected from the group consisting of:
- (i) a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 3 or the mature polypeptide of SEQ ID NO:
- 2;
- and
- (ii) a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 6 or the mature polypeptide of SEQ ID NO:
- 5.
An isolated host cell, comprising a polynucleotide encoding a polypeptide operably linked to one or more control sequences that direct the production of the polypeptide;
- the polypeptide selected from the group consisting of:
- (i) a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 3 or the mature polypeptide of SEQ ID NO:
- 2;
- and
- (ii) a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 6 or the mature polypeptide of SEQ ID NO:
- 5.
Dependencies are inferred from the claim text.