Water soluble fluorescent or colored dyes and methods for their use
AssigneesSony Corporation, Sony Corporation of America
InventorsTracy Matray, Hesham Sherif
Life of this patent
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Abstract
Compounds useful as fluorescent or colored dyes are disclosed. The compounds have the following structure (I): including stereoisomers, salts and tautomers thereof, wherein R1a, R1b, R2a, R2b, R2c, R2d, R2e, R2f, R2g, R2h, R2i, R2j, R2k, R2l, R2m, R2n, R2o, R2p, R2q, R2r and R2s are as defined herein. Methods associated with preparation and use of such compounds are also provided.
Claims8
A method of staining a sample, the method comprising adding to the sample an analyte molecule in an amount sufficient to produce an optical response when said sample is illuminated at an appropriate wavelength,
- wherein:
- the analyte molecule has the following structure (I′):
- or a salt thereof, wherein:
- R1a and R1b are each independently hydroxyl or alkoxy;
- and
- R2a, R2b, R2c, R2d, R2e, R2f, R2g, R2h, R2i, R2j, R2k, R2l, R2m, R2n, R2o, R2p, R2q, R2r, R2s and R2t are each independently H, halo or -L1-R3;
- R3 is, at each occurrence, independently a nucleic acid, peptide, carbohydrate, lipid, enzyme, receptor, receptor ligand, antibody, glycoprotein, aptamer, antigen, prion, drug, vitamin or small molecule;
- and
- L1 is an optional linker moiety,
- wherein at least one of R2a, R2b, R2c, R2d, R2e, R2f, R2g, R2h, R2i, R2j, R2k, R2l, R2m, R2n, R2o, R2p, R2q, R2r, R2s or R2t is -L1-R3.
A method for visually detecting an analyte molecule, the method comprising:
- (a) providing the analyte molecule;
- and
- (b) detecting the analyte molecule by its visible properties
- wherein:
- the analyte molecule has the following structure (I′):
- or a salt thereof, wherein:
- R1a and R1b are each independently hydroxyl or alkoxy;
- and
- R2a, R2b, R2c, R2d, R2e, R2f, R2g, R2h, R2i, R2j, R2k, R2l, R2m, R2n, R2o, R2p, R2q, R2r, R2s and R2t are each independently H, halo or -L1-R3;
- R3 is, at each occurrence, independently a nucleic acid, peptide, carbohydrate, lipid, enzyme, receptor, receptor ligand, antibody, glycoprotein, aptamer, antigen, prion, drug, vitamin or small molecule;
- and
- L1 is an optional linker moiety,
- wherein at least one of R2a, R2b, R2c, R2d, R2e, R2f, R2g, R2h, R2i, R2j, R2k, R2l, R2m, R2n, R2o, R2p, R2q, R2r, R2s or R2t is -L1-R3.
A method for visually detecting a biomolecule, the method comprising:
- (a) admixing a compound with one or more biomolecules;
- and
- (b) detecting the compound by its visible properties
- wherein:
- the compound has the following structure (I):
- or a salt, stereoisomer or tautomer thereof, wherein:
- R1a and R1b are each independently hydroxyl or alkoxy;
- and
- R2a, R2b, R2c, R2d, R2e, R2f, R2g, R2h, R2i, R2j, R2k, R2l, R2m, R2n, R2o, R2p, R2q, R2r, R2s and R2t are each independently H, halo or -L1-R3;
- R3 is, at each occurrence, independently the biomolecule, an alkyl substituted with one or more of hydroxyl, protected hydroxyl, amino, alkylamino, alkoxy, polyalkyether, hydroxylalkoxy, aminoalkoxy, hydroxylpolyalkyether, aminopolyalkyether, phosphate, thiophosphate, phospho, thiophospho, phosphoalkylether, Ophosphoalkylether, thiophosphoalkylether, Othiophosphoalkylether, phosphoramidite or activated phosphorous;
- or R3 is a microparticle;
- and
- L1 is an optional linker moiety.
Dependencies are inferred from the claim text.