Cellobiohydrolase variants and polynucleotides encoding same

An isolated variant of a parent cellobiohydrolase, comprising a substitution at one or more positions corresponding to positions 112, 154, 197, 228, 261, 306, and 375 of the mature polypeptide of SEQ ID NO:

• 2, wherein the substitution at position 112 is with His, the substitution at position 154 is with Met, the substitution at position 197 is with Tyr, the substitution at position 228 is with Val, the substitution at position 261 is with Leu, the substitution at position 306 is with Ala, and the substitution at position 375 is with Glu, wherein the variant has cellobiohydrolase activity and an increased thermostability compared to the parent polypeptide, and wherein the parent cellobiohydrolase is selected from the group consisting of:
• (a) a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
• 2, SEQ ID NO:
• 4, SEQ ID NO:
• 6, SEQ ID NO:
• 8, SEQ ID NO:
• 10, SEQ ID NO:
• 12, SEQ ID NO:
• 14, SEQ ID NO:
• 16, SEQ ID NO:
• 18, SEQ ID NO:
• 20, SEQ ID NO:
• 22, SEQ ID NO:
• 24, SEQ ID NO:
• 26, SEQ ID NO:
• 28, SEQ ID NO:
• 30, SEQ ID NO:
• 32, SEQ ID NO:
• 34, SEQ ID NO:
• 36, SEQ ID NO:
• 38, SEQ ID NO:
• 40, SEQ ID NO:
• 42, SEQ ID NO:
• 44, SEQ ID NO:
• 46, SEQ ID NO:
• 48, SEQ ID NO:
• 50, SEQ ID NO:
• 52, SEQ ID NO:
• 54, SEQ ID NO:
• 56, SEQ ID NO:
• 58, SEQ ID NO:
• 60, SEQ ID NO:
• 62, SEQ ID NO:
• 64, SEQ ID NO:
• 66, SEQ ID NO:
• 68, SEQ ID NO:
• 70, SEQ ID NO:
• 110, SEQ ID NO:
• 112, SEQ ID NO:
• 114, or SEQ ID NO:
• 116;
• (b) a polypeptide encoded by a polynucleotide having at least 98% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 1, SEQ ID NO:
• 3, SEQ ID NO:
• 5, SEQ ID NO:
• 7, SEQ ID NO:
• 9, SEQ ID NO:
• 11, SEQ ID NO:
• 13, SEQ ID NO:
• 15, SEQ ID NO:
• 17, SEQ ID NO:
• 19, SEQ ID NO:
• 21, SEQ ID NO:
• 23, SEQ ID NO:
• 25, SEQ ID NO:
• 27, SEQ ID NO:
• 29, SEQ ID NO:
• 31, SEQ ID NO:
• 33, SEQ ID NO:
• 35, SEQ ID NO:
• 37, SEQ ID NO:
• 39, SEQ ID NO:
• 41, SEQ ID NO:
• 43, SEQ ID NO:
• 45, SEQ ID NO:
• 47, SEQ ID NO:
• 49, SEQ ID NO:
• 51, SEQ ID NO:
• 53, SEQ ID NO:
• 55, SEQ ID NO:
• 57, SEQ ID NO:
• 59, SEQ ID NO:
• 61, SEQ ID NO:
• 63, SEQ ID NO:
• 65, SEQ ID NO:
• 67, SEQ ID NO:
• 69, SEQ ID NO:
• 109, SEQ ID NO:
• 111, SEQ ID NO:
• 113, or SEQ ID NO:
• 115;
• and (c) a fragment of the mature polypeptide of SEQ ID NO:
• 2, SEQ ID NO:
• 4, SEQ ID NO:
• 6, SEQ ID NO:
• 8, SEQ ID NO:
• 10, SEQ ID NO:
• 12, SEQ ID NO:
• 14, SEQ ID NO:
• 16, SEQ ID NO:
• 18, SEQ ID NO:
• 20, SEQ ID NO:
• 22, SEQ ID NO:
• 24, SEQ ID NO:
• 26, SEQ ID NO:
• 28, SEQ ID NO:
• 30, SEQ ID NO:
• 32, SEQ ID NO:
• 34, SEQ ID NO:
• 36, SEQ ID NO:
• 38, SEQ ID NO:
• 40, SEQ ID NO:
• 42, SEQ ID NO:
• 44, SEQ ID NO:
• 46, SEQ ID NO:
• 48, SEQ ID NO:
• 50, SEQ ID NO:
• 52, SEQ ID NO:
• 54, SEQ ID NO:
• 56, SEQ ID NO:
• 58, SEQ ID NO:
• 60, SEQ ID NO:
• 62, SEQ ID NO:
• 64, SEQ ID NO:
• 66, SEQ ID NO:
• 68, SEQ ID NO:
• 70, SEQ ID NO:
• 110, SEQ ID NO:
• 112, SEQ ID NO:
• 114, or SEQ ID NO:
• 116, which has cellobiohydrolase activity.