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US 10,036,047 B2Granted
Methods for hydroxylating phenylpropanoids
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsYajun Yan, Yuheng Lin
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Abstract
Provided herein are methods for producing an ortho-hydroxylated phenylpropanoid. In one embodiment the method includes culturing a microbe that includes HpaBC activity in the presence of a phenylpropanoid substrate. Also provided are genetically engineered microbes engineered to have greater levels of HpaB and/or HpaC than a control microbe.
Claims21
A method for producing an ortho-hydroxylated phenylpropanoid comprising:
- culturing a microbe comprising HpaBC activity in the presence of a phenylpropanoid substrate under conditions suitable to ortho-hydroxylate the phenylpropanoid substrate to result in an ortho-hydroxylated phenylpropanoid,
- wherein the microbe comprises coding regions encoding HpaB and HpaC, the HpaB comprising an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO:1, and the HpaC comprising an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO:2,
- wherein the phenylpropanoid substrate comprises a first six-carbon ring and at least one additional cyclic structure, and wherein the phenylpropanoid substrate comprises a structure having the formula
- wherein R1 is the position at which the phenylpropanoid substrate is ortho-hydroxylated by HpaBC, and
- wherein R2 is a divalent organic linking group that comprises the additional cyclic structure and wherein R3 is a hydrogen or a nonring substituent, or
- wherein R2 is joined to R3 to form the additional cyclic structure;
- and
- isolating the ortho-hydroxylated phenylpropanoid from the microbe or the culture medium, or the combination thereof.
5 more elements
Dependencies are inferred from the claim text.