Alkenol dehydratase variants of linalool dehydratase-isomerase
AssigneesGlobal Bioenergies, Scientist of Fortune, S.A.
InventorsPhilippe Marliere, Marc Delcourt, Sabine Mazaleyrat
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Abstract
Described are alkenol dehydratase variants having improved activity in catalyzing the conversion of a compound corresponding to the general formula CnH2nO into CnH2n-2 +H2O with 3<n<7. In particular, described are alkenol dehydratase variants having, e.g., an improved activity in converting but-2-en-1-ol (crotyl alcohol) into 1,3 butadiene and/or an improved activity in converting but-3-en-2-ol into 1,3 butadiene and/or an improved activity in converting 3-methylbut-2-en-1-ol (prenol) or 3-methylbut-3-en-2-ol (isoprenol) into isoprene and/or an improved activity in converting 2,3-dimethyl-but-2-en-1-ol into dimethyl-butadiene.
Claims26
Linalool dehydratase-isomerase variant having an amino acid sequence at least 80% sequence identity to SEQ ID NO:1, wherein said variant is capable of converting at least one alkenol compound corresponding to the general formula CnH2nO into a conjugated diene CnH2n-2 with an improved activity over the activity of the amino acid sequence from which the variant is derived, wherein 3<n<7,
- and wherein said variant
- (a) is characterized in that it is capable of converting crotyl alcohol into 1,3 butadiene with a turnover rate of at least 0.033×10−3 s−1 of crotyl alcohol into 1,3 butadiene;
- or
- (b) is characterized in that it is capable of converting but-3-en-2-ol into 1,3 butadiene with a turnover rate of at least 1.1×10−4 s−1 of but-3-en-2-ol into 1,3 butadiene;
- or
- (c) is characterized in that it is capable of converting-prenol into isoprene with a turnover rate of at least 3.3×10−4 s−1 of prenol into isoprene;
- or
- (d) is characterized in that it is capable of converting isoprenol into isoprene with a turnover rate of at least 3.3×10−5 s−1 of isoprenol into isoprene.
Dependencies are inferred from the claim text.