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US 10,035,991 B2Granted
Non-transgenic herbicide resistant plants
AssigneeCibus US LLC
InventorsPeter R. Beetham, Patricia L. Avissar, Keith A. Walker, Richard A. Metz
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AssigneeCibus US LLC
InventorsPeter R. Beetham, Patricia L. Avissar, Keith A. Walker, Richard A. Metz
Estimating the term…
The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. The present invention also relates to a non-transgenic plant cell in which the EPSPS gene has been mutated, a non-transgenic plant regenerated therefrom, as well as a plant resulting from a cross using a regenerated non-transgenic plant having a mutated EPSPS gene.
A non-transgenic herbicide resistant plant which comprises a plant that expresses a mutant EPSPS gene product instead of a wild-type EPSPS gene product instead of a wild-type EPSPS gene product wherein the wild-type EPSPS gene product has been mutated at one or more amino acid positions, said positions selected from the group consisting of Leu173, Ala179, Met180, Arg181, Ser98, Ser255 and Leu198 in SEQ ID NO:2 or at an analogous amino acid residue in an EPSPS paralog and said plant has substantially normal growth as compared to a plant expressing the wild-type EPSPS gene product;
A non-transgenic herbicide resistant plant which comprises a plant that expresses a mutant EPSPS gene product instead of a wild-type EPSPS gene product instead of a wild-type EPSPS gene product wherein the wild-type EPSPS gene product has been mutated at one or more amino acid positions, said positions selected from the group consisting of Leu173, Ala179, Met180, Arg181, Ser98, Ser255 and Leu198 in SEQ ID NO:2 or at an analogous amino acid residue in an EPSPS paralog and said plant has substantially the same catalytic activity as compared to a plant expressing the wild-type EPSPS gene product;
A non-transgenic herbicide resistant plant which comprises a plant that expresses a mutant EPSPS gene product instead of a wild-type EPSPS gene product instead of a wild-type EPSPS gene product wherein the wild-type EPSPS gene product has been mutated at one or more amino acid positions, said positions selected from the group consisting of Leu173, Ala179, Met180, Arg181, Ser98, Ser255 and Leu198 in Arabidopsis or at an analogous amino acid residue in an EPSPS paralog and said plant has substantially normal growth as compared to a plant expressing the wild-type EPSPS gene product;
A non-transgenic herbicide resistant plant which comprises a plant that expresses a mutant EPSPS gene product instead of a wild-type EPSPS gene product instead of a wild-type EPSPS gene product wherein the wild-type EPSPS gene product has been mutated at one or more amino acid positions, said positions selected from the group consisting of Leu173, Ala179, Met180, Arg181, Ser98, Ser255 and Leu198 in Arabidopsis or at an analogous amino acid residue in an EPSPS paralog and said plant has substantially the same catalytic activity as compared to a plant expressing the wild-type EPSPS gene product;
Dependencies are inferred from the claim text.