AgsE-deficient strain
AssigneeDsm IP Assets B.V.
InventorsNoël Nicolaas Maria Elisabeth Van Peij, Martina Beishuizen, Peter Jozef Ida Van De Vondervoort
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Abstract
The present invention relates to a mutant microbial host cell which is deficient in the production of the AgsE protein or in the production of an homologous thereof if compared with a parent microbial host cell which has not been modified and measured under the same conditions. It has been surprisingly found that when the mutant microbial host cell according to the invention is used in a method to produce a compound of interest, for example an enzyme, an improved yield of said compound is obtained if compared to a method in which a parent host cell which has not been modified is used when measured under the same conditions.
Claims14
A mutant filamentous fungal host cell which has been modified in the genome thereof to result in a deficiency in production of a polypeptide selected from the group consisting of:
- a. a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 3 or a polypeptide at least 90% identical thereto and having an activity of the amino acid sequence of SEQ ID NO:
- 3;
- b. a mature polypeptide comprising the amino acid sequence of SEQ ID NO:
- 4 or a polypeptide at least 90% identical thereto and having an activity of the mature polypeptide;
- and
- c. a polypeptide encoded by the polynucleotide sequence of SEQ ID NO:
- 1 or 2 or encoded by a polynucleotide at least 90% identical to SEQ ID NO:
- 1 or 2, wherein said polypeptide encoded by a polynucleotide at least 90% identical to SEQ ID NO:
- 1 or 2 has an activity of the polypeptide encoded by the polynucleotide sequence of SEQ ID NO:
- 1 or 2;
- compared with a parent filamentous fungal host cell which has not been modified and measured under the same conditions;
- wherein the modification in the genome of the mutant filamentous fungal host cell is selected from:
- i) a full or partial deletion of SEQ ID NO:
- 1 or a polynucleotide at least 90% identical to SEQ ID NO:
- 1;
- ii) a full or partial replacement of SEQ ID NO:
- 1 or a polynucleotide at least 90% identical to SEQ ID NO:
- 1 with a polynucleotide sequence which does not code for a polypeptide as defined in a to c or which codes for a partially or fully inactive form of a polypeptide as defined in a to c;
- and
- iii) a disruption of SEQ ID NO:
- 1 or a polynucleotide at least 90% identical to SEQ ID NO:
- 1 by the insertion of one or more nucleotides in the polynucleotide sequence, wherein the disruption results in partial or full inactivation of a polypeptide as defined in a to c;
- wherein the mutant filamentous fungal host cell further comprises at least one recombinant polynucleotide coding for an enzyme of interest or at least one recombinant polynucleotide coding for a polypeptide involved in production of a metabolite of interest, wherein the enzyme of interest or the metabolite of interest is a compound which can be produced and isolated from the host cell on an industrial scale and
- wherein the activity of the polypeptide defined by a to c is an enzymatic activity which is α-1,3-glucan synthase activity [EC 2.4.1.183].
Dependencies are inferred from the claim text.