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US 10,030,229 B2Granted
SC-β cells and compositions and methods for generating the same
AssigneePresident and Fellows of Harvard College
InventorsQuinn P. Peterson, Felicia J. Pagliuca, Douglas A. Melton, Jeffrey R. Millman, Michael Saris Segel, Mads Gurtler
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Abstract
Disclosed herein are methods, compositions, kits, and agents useful for inducing β cell maturation, and isolated populations of SC-β cells for use in various applications, such as cell therapy.
Claims22
A composition comprising a non-native pancreatic βcell, wherein:
- (a) the non-native pancreatic βcell comprises one or more crystalline insulin granules;
- (b) the non-native pancreatic βcell expresses the following genes:
- INS, PDX1, NKX6-1, and ZNT8;
- (c) the non-native pancreatic βcell exhibits an in vitro glucose-stimulated insulin secretion response to a first glucose challenge;
- and
- (d) the non-native pancreatic βcell does not express one or both of somatostatin and glucagon;
- and wherein said composition is in the form of an in vitro cell cluster.
4 more elements
Dependencies are inferred from the claim text.