Device for the microstructured grafting of proteins onto a substrate

A device for the microstructured grafting of several proteins onto a substrate, comprising:

• a substrate,
• a layer comprising a polyethylene glycol and being placed on the substrate,
• a matrix for propagating the light in a first structured pattern and for replacing the first structured pattern with a second structured pattern,
• a light source for illuminating the matrix,
• an optical system for forming, on the layer, a first two-dimensional microstructured image of the first structured pattern and a second two-dimensional microstructured image of the second structured pattern,
• a first container for receiving a first aqueous solution comprising a first grafting component and a first protein,
• a second container for receiving a second aqueous solution comprising a second grafting component and a second protein, and
• a microfluidic circuit for containing the first aqueous solution, comprising an opening for bringing the first aqueous solution into contact with the layer at the opening,
• wherein the microfluidic circuit is adapted to be filled with the first aqueous solution so as to bring the first aqueous solution and the layer into contact, the first two-dimensional microstructured image of the first structured pattern being formed on the layer by means of the light source in order to photoprint the first protein on the layer,
• wherein the microfluidic circuit is adapted to replace the first aqueous solution with the second aqueous solution so as to bring the second aqueous solution and the layer into contact, the first structured pattern being replaced with the second structured pattern so as to form the second two-dimensional microstructured image of the second structured pattern on the layer by means of the light source in order to photoprint the second protein on the layer, and
• wherein said matrix is a planar two-dimensional matrix of micromirrors, each micromirror being individually controlled and propagating the light by reflection, so that the first and second two-dimensional microstructured images have a number of pixels equal to the number of micromirrors of the matrix.